{"doi":"10.1016/j.celrep.2020.107832","title":"Discovering How Heme Controls Genome Function Through Heme-omics","abstract":"Protein ensembles control genome function by establishing, maintaining, and deconstructing cell-type-specific chromosomal landscapes. A plethora of small molecules orchestrate cellular functions and therefore may link physiological processes with genome biology. The metabolic enzyme and hemoglobin cofactor heme induces proteolysis of a transcriptional repressor, Bach1, and regulates gene expression post-transcriptionally. However, whether heme controls genome function broadly or through prescriptive actions is unclear. Using assay for transposase-accessible chromatin sequencing (ATAC-seq), we establish a heme-dependent chromatin atlas in wild-type and mutant erythroblasts lacking enhancers that confer normal heme synthesis. Amalgamating chromatin landscapes and transcriptomes in cells with sub-physiological heme and post-heme rescue reveals parallel Bach1-dependent and Bach1-independent mechanisms that target heme-sensing chromosomal hotspots. The hotspots harbor a DNA motif demarcating heme-regulated chromatin and genes encoding proteins not known to be heme regulated, including metabolic enzymes. The heme-omics analysis establishes how an essential biochemical cofactor controls genome function and cellular physiology.","journal":"Cell Reports","year":2020,"id":63050,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":47,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9479,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":333663,"name":"Ye Zheng","orcid":"0000-0002-8806-2761","position":1,"is_corresponding":false},{"id":299105,"name":"Xin Liu","orcid":"0000-0002-5646-423X","position":2,"is_corresponding":false},{"id":237855,"name":"Yuannyu Zhang","orcid":"0000-0002-7016-7557","position":3,"is_corresponding":false},{"id":333664,"name":"Gretchen Seim","orcid":"0000-0002-7785-1378","position":4,"is_corresponding":false},{"id":335449,"name":"Nobuyuki Tanimura","orcid":null,"position":5,"is_corresponding":false},{"id":292904,"name":"Gary M. Wilson","orcid":"0000-0001-6376-273X","position":6,"is_corresponding":false},{"id":277447,"name":"Peiman Hematti","orcid":"0000-0001-5836-4497","position":7,"is_corresponding":false},{"id":71208,"name":"Joshua J. Coon","orcid":"0000-0002-0004-8253","position":8,"is_corresponding":false},{"id":333665,"name":"Jing Fan","orcid":"0000-0002-5326-5358","position":9,"is_corresponding":false},{"id":56479,"name":"Jian Xu","orcid":"0000-0003-1988-7337","position":10,"is_corresponding":false},{"id":43259,"name":"Sündüz Keleş","orcid":"0000-0001-9048-0922","position":11,"is_corresponding":false},{"id":333666,"name":"Emery H. Bresnick","orcid":"0000-0002-1151-5654","position":12,"is_corresponding":false},{"id":333662,"name":"Ruiqi Liao","orcid":"0000-0002-9553-3349","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":null,"created_at":"2026-07-18T21:11:00.632961Z","pmid":"32610133","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}