{"doi":"10.1016/j.celrep.2020.107693","title":"MKRN2 Physically Interacts with GLE1 to Regulate mRNA Export and Zebrafish Retinal Development","abstract":"The mammalian mRNA nuclear export process is thought to terminate at the cytoplasmic face of the nuclear pore complex through ribonucleoprotein remodeling. We conduct a stringent affinity-purification mass-spectrometry-based screen of the physical interactions of human RNA-binding E3 ubiquitin ligases. The resulting protein-interaction network reveals interactions between the RNA-binding E3 ubiquitin ligase MKRN2 and GLE1, a DEAD-box helicase activator implicated in mRNA export termination. We assess MKRN2 epistasis with GLE1 in a zebrafish model. Morpholino-mediated knockdown or CRISPR/Cas9-based knockout of MKRN2 partially rescue retinal developmental defects seen upon GLE1 depletion, consistent with a functional association between GLE1 and MKRN2. Using ribonomic approaches, we show that MKRN2 binds selectively to the 3' UTR of a diverse subset of mRNAs and that nuclear export of MKRN2-associated mRNAs is enhanced upon knockdown of MKRN2. Taken together, we suggest that MKRN2 interacts with GLE1 to selectively regulate mRNA nuclear export and retinal development.","journal":"Cell Reports","year":2020,"id":69778,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9494,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":369420,"name":"Amanda Miles","orcid":"0000-0003-2541-2936","position":1,"is_corresponding":false},{"id":370455,"name":"Eliza S. Lee","orcid":null,"position":2,"is_corresponding":false},{"id":369421,"name":"Syed Nabeel‐Shah","orcid":"0000-0002-4239-7443","position":3,"is_corresponding":false},{"id":43252,"name":"Jack Greenblatt","orcid":"0000-0003-1762-2861","position":4,"is_corresponding":false},{"id":237124,"name":"Alexander F. Palazzo","orcid":"0000-0002-9700-1995","position":5,"is_corresponding":false},{"id":106264,"name":"Vincent Tropepe","orcid":"0000-0001-8611-5746","position":6,"is_corresponding":false},{"id":253656,"name":"Andrew Emili","orcid":"0000-0001-8995-246X","position":7,"is_corresponding":false},{"id":369419,"name":"Eric J. Wolf","orcid":"0009-0005-7262-2364","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-18T21:42:48.813846Z","pmid":"32460013","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}