{"doi":"10.1016/j.celrep.2020.107583","title":"HIV-1 Envelope and MPER Antibody Structures in Lipid Assemblies","abstract":"Structural and functional studies of HIV envelope glycoprotein (Env) as a transmembrane protein have long been complicated by challenges associated with inherent flexibility of the molecule and the membrane-embedded hydrophobic regions. Here, we present approaches for incorporating full-length, wild-type HIV-1 Env, as well as C-terminally truncated and stabilized versions, into lipid assemblies, providing a modular platform for Env structural studies by single particle electron microscopy. We reconstitute a full-length Env clone into a nanodisc, complex it with a membrane-proximal external region (MPER) targeting antibody 10E8, and structurally define the full quaternary epitope of 10E8 consisting of lipid, MPER, and ectodomain contacts. By aligning this and other Env-MPER antibody complex reconstructions with the lipid bilayer, we observe evidence of Env tilting as part of the neutralization mechanism for MPER-targeting antibodies. We also adapt the platform toward vaccine design purposes by introducing stabilizing mutations that allow purification of unliganded Env with a peptidisc scaffold.","journal":"Cell Reports","year":2020,"id":55313,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":94,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":230080,"name":"Zachary T. Berndsen","orcid":"0000-0003-4531-9907","position":1,"is_corresponding":false},{"id":230743,"name":"Aleksandar Antanasijevic","orcid":"0000-0001-9452-8954","position":2,"is_corresponding":false},{"id":285899,"name":"Torben Schiffner","orcid":"0000-0001-6772-8870","position":3,"is_corresponding":false},{"id":285900,"name":"Xi Zhang","orcid":"0000-0003-3599-4693","position":4,"is_corresponding":false},{"id":285901,"name":"Wen-Hsin Lee","orcid":"0000-0001-9445-6671","position":5,"is_corresponding":false},{"id":106545,"name":"Jonathan L. Torres","orcid":"0000-0003-0137-8497","position":6,"is_corresponding":false},{"id":285902,"name":"Lei Zhang","orcid":"0000-0002-7704-5237","position":7,"is_corresponding":false},{"id":287280,"name":"A. Irimia","orcid":null,"position":8,"is_corresponding":false},{"id":249506,"name":"Jeffrey Copps","orcid":"0000-0003-4793-7258","position":9,"is_corresponding":false},{"id":285903,"name":"Kenneth Zhou","orcid":"0000-0001-5295-2693","position":10,"is_corresponding":false},{"id":285904,"name":"Young Do Kwon","orcid":"0000-0002-1188-7458","position":11,"is_corresponding":false},{"id":285905,"name":"William H. Law","orcid":"0000-0003-3142-989X","position":12,"is_corresponding":false},{"id":283220,"name":"Chaim A. Schramm","orcid":null,"position":13,"is_corresponding":false},{"id":266886,"name":"Raffaello Verardi","orcid":"0000-0002-2613-1223","position":14,"is_corresponding":false},{"id":285906,"name":"Shelly J. Krebs","orcid":"0000-0003-1136-1760","position":15,"is_corresponding":false},{"id":105633,"name":"Peter D. Kwong","orcid":"0000-0003-3560-232X","position":16,"is_corresponding":false},{"id":105605,"name":"Nicole A. Doria‐Rose","orcid":"0000-0002-5731-3054","position":17,"is_corresponding":false},{"id":105430,"name":"Ian A. Wilson","orcid":"0000-0002-6469-2419","position":18,"is_corresponding":false},{"id":285907,"name":"Michael B. Zwick","orcid":"0000-0002-0885-2502","position":19,"is_corresponding":false},{"id":55408,"name":"John R. Yates","orcid":"0000-0001-5267-1672","position":20,"is_corresponding":false},{"id":236279,"name":"William R. Schief","orcid":"0000-0002-1120-0150","position":21,"is_corresponding":false},{"id":230082,"name":"Andrew B. Ward","orcid":"0000-0001-7153-3769","position":22,"is_corresponding":false},{"id":285898,"name":"Kimmo Rantalainen","orcid":"0000-0002-3158-9183","position":0,"is_corresponding":true}],"reference_count":82,"raw_metadata":null,"created_at":"2026-07-18T21:04:59.506324Z","pmid":"32348769","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}