{"doi":"10.1016/j.celrep.2020.02.061","title":"Mapping Polyclonal Antibody Responses in Non-human Primates Vaccinated with HIV Env Trimer Subunit Vaccines","abstract":"Rational immunogen design aims to focus antibody responses to vulnerable sites on primary antigens. Given the size of these antigens, there is, however, potential for eliciting unwanted, off-target responses. Here, we use our electron microscopy polyclonal epitope mapping approach to describe the antibody specificities elicited by immunization of non-human primates with soluble HIV envelope trimers and subsequent repeated viral challenge. An increased diversity of epitopes recognized and the approach angle by which these antibodies bind constitute a hallmark of the humoral response in most protected animals. We also show that fusion peptide-specific antibodies are likely responsible for some neutralization breadth. Moreover, cryoelectron microscopy (cryo-EM) analysis of a fully protected animal reveals a high degree of clonality within a subset of putatively neutralizing antibodies, enabling a detailed molecular description of the antibody paratope. Our results provide important insights into the immune response against a vaccine candidate that entered into clinical trials in 2019.","journal":"Cell Reports","year":2020,"id":54163,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":107,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9495,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":277779,"name":"Matteo Bianchi","orcid":"0000-0003-0517-8599","position":1,"is_corresponding":false},{"id":279923,"name":"Christopher A. Cottrell","orcid":null,"position":2,"is_corresponding":false},{"id":279924,"name":"Robert N. Kirchdoerfer","orcid":null,"position":3,"is_corresponding":false},{"id":277780,"name":"Leigh M. Sewall","orcid":"0000-0002-2020-2991","position":4,"is_corresponding":false},{"id":230742,"name":"Hannah L. Turner","orcid":"0000-0002-4745-8057","position":5,"is_corresponding":false},{"id":108971,"name":"Fangzhu Zhao","orcid":"0000-0002-3172-1340","position":6,"is_corresponding":false},{"id":108978,"name":"Devin Sok","orcid":"0000-0002-9354-1740","position":7,"is_corresponding":false},{"id":108980,"name":"Dennis R. Burton","orcid":"0000-0001-6711-9864","position":8,"is_corresponding":false},{"id":277781,"name":"Lars Hangartner","orcid":"0000-0002-3165-2058","position":9,"is_corresponding":false},{"id":230082,"name":"Andrew B. Ward","orcid":"0000-0001-7153-3769","position":10,"is_corresponding":false},{"id":277778,"name":"Bartek Nogal","orcid":"0000-0001-8977-7470","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-18T21:03:43.037640Z","pmid":"32187547","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}