{"doi":"10.1016/j.celrep.2020.01.073","title":"Glioma-Derived miRNA-Containing Extracellular Vesicles Induce Angiogenesis by Reprogramming Brain Endothelial Cells","abstract":"Glioblastoma (GBM) is characterized by aberrant vascularization and a complex tumor microenvironment. The failure of anti-angiogenic therapies suggests pathways of GBM neovascularization, possibly attributable to glioblastoma stem cells (GSCs) and their interplay with the tumor microenvironment. It has been established that GSC-derived extracellular vesicles (GSC-EVs) and their cargoes are proangiogenic in vitro. To further elucidate EV-mediated mechanisms of neovascularization in vitro, we perform RNA-seq and DNA methylation profiling of human brain endothelial cells exposed to GSC-EVs. To correlate these results to tumors in vivo, we perform histoepigenetic analysis of GBM molecular profiles in the TCGA collection. Remarkably, GSC-EVs and normal vascular growth factors stimulate highly distinct gene regulatory responses that converge on angiogenesis. The response to GSC-EVs shows a footprint of post-transcriptional gene silencing by EV-derived miRNAs. Our results provide insights into targetable angiogenesis pathways in GBM and miRNA candidates for liquid biopsy biomarkers.","journal":"Cell Reports","year":2020,"id":51493,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":154,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.945,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":257525,"name":"Valentina Zappulli","orcid":"0000-0001-6203-0190","position":1,"is_corresponding":false},{"id":257526,"name":"Alessandro Sammarco","orcid":"0000-0003-1977-3292","position":2,"is_corresponding":false},{"id":257527,"name":"Oscar Murillo","orcid":"0000-0003-1938-3905","position":3,"is_corresponding":false},{"id":257528,"name":"Pike-See Cheah","orcid":"0000-0003-2181-2502","position":4,"is_corresponding":false},{"id":257529,"name":"Srimeenakshi Srinivasan","orcid":"0000-0002-5045-7880","position":5,"is_corresponding":false},{"id":260847,"name":"Eric C. Tai","orcid":null,"position":6,"is_corresponding":false},{"id":43401,"name":"David T. Ting","orcid":"0000-0002-3261-2322","position":7,"is_corresponding":false},{"id":257530,"name":"Zhiyun Wei","orcid":"0000-0002-3554-4142","position":8,"is_corresponding":false},{"id":257531,"name":"Matthew E. Roth","orcid":"0000-0001-5235-3789","position":9,"is_corresponding":false},{"id":105299,"name":"Louise C. Laurent","orcid":"0000-0002-2095-7534","position":10,"is_corresponding":false},{"id":257532,"name":"Anna M. Krichevsky","orcid":"0000-0002-5080-5507","position":11,"is_corresponding":false},{"id":2731,"name":"Xandra O. Breakefield","orcid":"0000-0001-6036-0399","position":12,"is_corresponding":false},{"id":62231,"name":"Aleksandar Milosavljevic","orcid":"0000-0001-5482-2825","position":13,"is_corresponding":false},{"id":257524,"name":"Rocco Lucero","orcid":"0000-0002-7026-0313","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-18T20:41:30.009880Z","pmid":"32075753","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}