{"doi":"10.1016/j.celrep.2019.12.027","title":"T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection","abstract":"Human rhinoviruses cause the common cold and exacerbate chronic respiratory diseases. Although infection elicits neutralizing antibodies, these do not persist or cross-protect across multiple rhinovirus strains. To analyze rhinovirus-specific B cell responses in humans, we developed techniques using intact RV-A16 and RV-A39 for high-throughput high-dimensional single-cell analysis, with parallel assessment of antibody isotypes in an experimental infection model. Our approach identified T-bet+ B cells binding both viruses that account for ∼5% of CXCR5- memory B cells. These B cells infiltrate nasal tissue and expand in the blood after infection. Their rapid secretion of heterotypic immunoglobulin G (IgG) in vitro, but not IgA, matches the nasal antibody profile post-infection. By contrast, CXCR5+ memory B cells binding a single virus are clonally distinct, absent in nasal tissue, and secrete homotypic IgG and IgA, mirroring the systemic response. Temporal and spatial functions of dichotomous memory B cells might explain the ability to resolve infection while rendering the host susceptible to re-infection.","journal":"Cell Reports","year":2020,"id":103709,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":350270,"name":"Ronald B. Turner","orcid":"0000-0002-7090-3219","position":1,"is_corresponding":false},{"id":504805,"name":"Nicole Kirk","orcid":"0000-0002-3609-5502","position":2,"is_corresponding":false},{"id":350268,"name":"Lyndsey M. Muehling","orcid":"0000-0003-3203-3264","position":3,"is_corresponding":false},{"id":321686,"name":"Larry Borish","orcid":"0000-0002-8298-8184","position":4,"is_corresponding":false},{"id":321681,"name":"John W. Steinke","orcid":"0000-0003-4571-8473","position":5,"is_corresponding":false},{"id":386201,"name":"Spencer C. Payne","orcid":"0000-0001-9736-4561","position":6,"is_corresponding":false},{"id":352048,"name":"Paul W. Wright","orcid":null,"position":7,"is_corresponding":false},{"id":505648,"name":"Deborah F. Thacker","orcid":null,"position":8,"is_corresponding":false},{"id":505649,"name":"Sampo J. Lahtinen","orcid":null,"position":9,"is_corresponding":false},{"id":504806,"name":"Markus J. Lehtinen","orcid":"0000-0002-8091-400X","position":10,"is_corresponding":false},{"id":352047,"name":"Peter W. Heymann","orcid":null,"position":11,"is_corresponding":false},{"id":350271,"name":"Judith A. Woodfolk","orcid":"0000-0002-8915-4334","position":12,"is_corresponding":false},{"id":350269,"name":"Jacob D. Eccles","orcid":"0000-0002-4202-0355","position":0,"is_corresponding":true}],"reference_count":103,"raw_metadata":null,"created_at":"2026-07-18T22:42:48.999009Z","pmid":"31940481","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}