{"doi":"10.1016/j.celrep.2019.10.027","title":"Glycosylation of Specific Notch EGF Repeats by O-Fut1 and Fringe Regulates Notch Signaling in Drosophila","abstract":null,"journal":"Cell Reports","year":2019,"id":671165,"datarank":1.0267359140414345,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":0.4892080732730179,"self_endowment_contribution":0.5375278407684165,"citer_contribution":0.4892080732730179,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":24,"citers_with_citation_signal":22,"citers_with_endowment":22,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1753332,"name":"Beth M. 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Although Notch has 22 O-fucosylation sites, the biologically relevant sites affecting Notch activity during animal development in vivo in the presence or absence of Fringe are not known. Using a variety of assays, we find important roles in Drosophila Notch signaling for GlcNAc-fucose-O glycans on three sites: EGF8, EGF9, and EGF12. O-Fucose monosaccharide on EGF12 (in the absence of Fringe) is essential for Delta-mediated lateral inhibition in embryos. However, wing vein development depends on the addition of GlcNAc to EGF8 and EGF12 by Fringe, with a minor contribution from EGF9. Fringe modifications of EGF8 and EGF12 together prevent Notch from cis-inhibiting Serrate, thereby promoting normal wing margin formation. Our work shows the combinatorial and context-dependent roles of GlcNAc-fucose-O glycans on these sites in Drosophila Notch-ligand interactions.","is_dataset_classified":null,"base_score":3.58351893845611,"endowment":3.58351893845611,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31722217","pmcid":"PMC6866671","openalex_id":"https://openalex.org/W2988430482","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R35 GM130317","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM061126","title":null},{"funder_name":"NICHD NIH HHS","grant_id":"U54 HD083092","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM084135","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA125123","title":null},{"funder_name":"NIH HHS","grant_id":"P40 OD018537","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM084135-07","title":"Fine-Tuning the Notch 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Center"},{"funder_name":"National Institutes of Health","grant_id":"5R35GM130317-02","title":"Regulation of developmental signaling pathways by glycosylation and deglycosylation"},{"funder_name":"National Institutes of Health","grant_id":"2R35GM130317-06","title":"Roles of Glycosylation and Deglycosylation During Animal Development"},{"funder_name":"National Institute of General Medical Sciences","grant_id":"","title":null},{"funder_name":"Mizutani Foundation for 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