{"doi":"10.1016/j.celrep.2019.04.015","title":"Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics","abstract":null,"journal":"Cell Reports","year":2019,"id":661332,"datarank":0.6307038929086449,"base_score":4.204692619390966,"endowment":4.204692619390966,"self_citation_contribution":0.6307038929086449,"citation_network_contribution":0.0,"self_endowment_contribution":0.6307038929086449,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":66,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":248948,"name":"Amit Fulzele","orcid":null,"position":1,"is_corresponding":false},{"id":1726456,"name":"Reneé Higgins","orcid":null,"position":2,"is_corresponding":false},{"id":300538,"name":"Marilyn Leonard","orcid":null,"position":3,"is_corresponding":false},{"id":43218,"name":"Gene W. Yeo","orcid":"0000-0002-0799-6037","position":4,"is_corresponding":false},{"id":248001,"name":"Eric J. Bennett","orcid":"0000-0002-1201-3314","position":5,"is_corresponding":false},{"id":166311,"name":"Sebastian Markmiller","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics","abstract":"Stress granule (SG) formation is frequently accompanied by ubiquitin proteasome system (UPS) impairment and ubiquitylated protein accumulation. SGs, ubiquitin, and UPS components co-localize, but the relationship between the ubiquitin pathway and SGs has not been systematically characterized. We utilize pharmacological inhibition of either the ubiquitin- or NEDD8-activating enzyme (UAE or NAE) to probe whether active ubiquitylation or neddylation modulate SG dynamics. We show that UAE inhibition results in rapid loss of global protein ubiquitylation using ubiquitin-specific proteomics. Critically, inhibiting neither UAE nor NAE significantly affected SG formation or disassembly, indicating that active protein ubiquitylation or neddylation is dispensable for SG dynamics. Using antibodies with varying preference for free ubiquitin or polyubiquitin and fluorescently tagged ubiquitin variants in combination with UAE inhibition, we show that SGs co-localize primarily with unconjugated ubiquitin rather than polyubiquitylated proteins. These findings clarify the role of ubiquitin in SG biology and suggest that free ubiquitin may alter SG protein interactions.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31042464","pmcid":"PMC6508666","openalex_id":null,"authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM127681","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"DP2 GM119132","title":null},{"funder_name":"NHGRI NIH HHS","grant_id":"R01 HG004659","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"R01 NS103172","title":null},{"funder_name":"National Institutes of Health","grant_id":"1DP2GM119132-01","title":"Manipulating Protein Homeostasis through Specialized Quality Control Ribosomes"},{"funder_name":"ALS Association","grant_id":"","title":null},{"funder_name":"Ionis Pharmaceuticals","grant_id":"","title":null},{"funder_name":"Larry L. 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