{"doi":"10.1016/j.celrep.2016.12.060","title":"Single-Cell Transcriptomic Analysis Defines Heterogeneity and Transcriptional Dynamics in the Adult Neural Stem Cell Lineage","abstract":null,"journal":"Cell Reports","year":2017,"id":632107,"datarank":8.811514759952033,"base_score":5.8664680569332965,"endowment":5.8664680569332965,"self_citation_contribution":0.8799702085399946,"citation_network_contribution":7.931544551412038,"self_endowment_contribution":0.8799702085399946,"citer_contribution":7.931544551412038,"corpus_percentile":null,"corpus_rank":null,"citation_count":352,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":783797,"name":"Dena S. Leeman","orcid":"0000-0003-4338-5433","position":1,"is_corresponding":false},{"id":98214,"name":"Stéphane C. Boutet","orcid":null,"position":2,"is_corresponding":false},{"id":567353,"name":"Katja Hebestreit","orcid":"0000-0002-6404-9917","position":3,"is_corresponding":false},{"id":14927,"name":"Anne Brunet","orcid":"0000-0002-4608-6845","position":4,"is_corresponding":false},{"id":1205707,"name":"Ben W. Dulken","orcid":"0000-0001-5042-3712","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Single-Cell Transcriptomic Analysis Defines Heterogeneity and Transcriptional Dynamics in the Adult Neural Stem Cell Lineage","abstract":"Neural stem cells (NSCs) in the adult mammalian brain serve as a reservoir for the generation of new neurons, oligodendrocytes, and astrocytes. Here, we use single-cell RNA sequencing to characterize adult NSC populations and examine the molecular identities and heterogeneity of in vivo NSC populations. We find that cells in the NSC lineage exist on a continuum through the processes of activation and differentiation. Interestingly, rare intermediate states with distinct molecular profiles can be identified and experimentally validated, and our analysis identifies putative surface markers and key intracellular regulators for these subpopulations of NSCs. Finally, using the power of single-cell profiling, we conduct a meta-analysis to compare in vivo NSCs and in vitro cultures, distinct fluorescence-activated cell sorting strategies, and different neurogenic niches. These data provide a resource for the field and contribute to an integrative understanding of the adult NSC lineage.","is_dataset_classified":null,"base_score":5.8664680569332965,"endowment":5.8664680569332965,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28099854","pmcid":"PMC5269583","openalex_id":"https://openalex.org/W2578839378","authors":[],"funders":[{"funder_name":"NIH Training Grant","grant_id":"T32 GM7365","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM007365","title":null},{"funder_name":"NIA NIH HHS","grant_id":"P01 AG036695","title":null},{"funder_name":"NCI NIH HHS","grant_id":"T32 CA009302","title":null},{"funder_name":"NIA NIH HHS","grant_id":"R01 AG031198","title":null},{"funder_name":"NIA NIH HHS","grant_id":"R01 AG056290","title":null},{"funder_name":"NIA NIH HHS","grant_id":"DP1 AG044848","title":null},{"funder_name":"NIA NIH HHS","grant_id":"T32 AG047126","title":null},{"funder_name":"National Institutes of Health","grant_id":"5P01AG036695-05","title":"Genomics and Ultra-High-Throughput Sequencing Core"},{"funder_name":"Stanford MSTP program","grant_id":"","title":null}],"total_grants":10,"fwci":14.3985,"citation_percentile":0.99385554,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2017,"count":14},{"year":2018,"count":41},{"year":2019,"count":51},{"year":2020,"count":36},{"year":2021,"count":50},{"year":2022,"count":37},{"year":2023,"count":37},{"year":2024,"count":41},{"year":2025,"count":35},{"year":2026,"count":9}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"http://www.cell.com/article/S2211124716317673/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S2211124716317673/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2211124716317673?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2211124716317673?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.celrep.2016.12.060","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28099854","host_type":"repository"},{"url":"https://doaj.org/article/047a1413bbf24976b45be163fd75544a","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5269583","host_type":"repository"},{"url":"http://dx.doi.org/10.1016/j.celrep.2016.12.060","host_type":""},{"url":"https://dx.doi.org/10.1016/j.celrep.2016.12.060","host_type":""}],"fields_of_study":["Single-cell and spatial transcriptomics","Neurogenesis and neuroplasticity mechanisms","Neuroinflammation and Neurodegeneration Mechanisms","0301 basic medicine","03 medical and health sciences","0303 health sciences","Algorithms","Animals","Astrocytes","Cell Differentiation","Cell Lineage","Connexin 43","Homeodomain Proteins","Ki-67 Antigen","Mice","Neural Stem Cells","Neurogenesis","Principal Component Analysis","RNA","Sequence Analysis, RNA","Single-Cell Analysis","Sodium-Potassium-Exchanging ATPase","Transcription Factors","Transcriptome"],"mesh_terms":["Sodium-Potassium-Exchanging ATPase","Algorithms","Animals","Astrocytes","Cell Differentiation","RNA","Transcription Factors","Sequence Analysis, RNA","Connexin 43","Homeodomain Proteins","Cell Lineage","Ki-67 Antigen","Principal Component Analysis","Mice","Neurogenesis","Neural Stem Cells","Single-Cell Analysis","Transcriptome"],"keywords":["Neural stem cell","Biology","Cell sorting","Transcriptome","Lineage (genetic)","Stem cell","Cell biology","Cell","Neural cell","Neurosphere","Cellular differentiation","Computational biology","Adult stem cell","Genetics","Gene","Gene expression","Neural stem cells","Machine Learning","Adult Neurogenesis","Single-cell Rna-seq","Pseudotemporal Ordering","QH301-705.5","Neurogenesis","Mice","Animals","Cell Lineage","Biology (General)","Homeodomain Proteins","Principal Component Analysis","Sequence Analysis, RNA","Cell Differentiation","Ki-67 Antigen","Astrocytes","Connexin 43","RNA","Single-Cell Analysis","Sodium-Potassium-Exchanging ATPase","Algorithms","Transcription Factors"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"bioproject"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T02:07:38.157662Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}