{"doi":"10.1016/j.cell.2024.12.015","title":"Designed mosaic nanoparticles enhance cross-reactive immune responses in mice","abstract":"Nanoparticle vaccines displaying combinations of SARS-like betacoronavirus (sarbecovirus) receptor-binding domains (RBDs) could protect against SARS-CoV-2 variants and spillover of zoonotic sarbecoviruses into humans. Using a computational approach, we designed variants of SARS-CoV-2 RBDs and selected 7 natural sarbecovirus RBDs, each predicted to fold properly and abrogate antibody responses to variable epitopes. RBDs were attached to 60-mer nanoparticles to make immunogens displaying two (mosaic-2 COM s), five (mosaic-5 COM ), or seven (mosaic-7 COM ) different RBDs for comparisons with mosaic-8b, which elicited cross-reactive antibodies and protected animals from sarbecovirus challenges. Naive and COVID-19 pre-vaccinated mice immunized with mosaic-7 COM elicited antibodies targeting conserved RBD epitopes, and their sera exhibited higher binding and neutralization titers against sarbecoviruses than mosaic-8b. Mosaic-2 COM s and mosaic-5 COM elicited higher antibody potencies against some SARS-CoV-2 variants than mosaic-7 COM . However, mosaic-7 COM elicited more potent responses against zoonotic sarbecoviruses and highly mutated Omicrons, supporting its use to protect against SARS-CoV-2 variants and zoonotic sarbecoviruses.","journal":"Cell","year":2025,"id":509883,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9541,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":16982,"name":"Alexander A. Cohen","orcid":"0000-0002-2818-656X","position":1,"is_corresponding":false},{"id":1064499,"name":"Luis F. Caldera","orcid":"0009-0005-3254-6563","position":2,"is_corresponding":false},{"id":16970,"name":"Jennifer R. Keeffe","orcid":"0000-0002-5317-6398","position":3,"is_corresponding":false},{"id":1204088,"name":"Annie V. Rorick","orcid":"0009-0003-1801-6940","position":4,"is_corresponding":false},{"id":1365085,"name":"Yusuf M Adia","orcid":"0009-0001-6538-0919","position":5,"is_corresponding":false},{"id":16983,"name":"Priyanthi N.P. Gnanapragasam","orcid":null,"position":6,"is_corresponding":false},{"id":17000,"name":"Pamela J. Björkman","orcid":"0000-0002-2277-3990","position":7,"is_corresponding":false},{"id":110109,"name":"Arup K. Chakraborty","orcid":"0000-0003-1268-9602","position":8,"is_corresponding":false},{"id":308075,"name":"Eric Wang","orcid":"0000-0003-0330-0597","position":0,"is_corresponding":true}],"reference_count":88,"raw_metadata":null,"created_at":"2026-07-19T02:47:30.942539Z","pmid":"39855201","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}