{"doi":"10.1016/j.cell.2024.08.044","title":"Intracellular Ebola virus nucleocapsid assembly revealed by in situ cryo-electron tomography","abstract":"Filoviruses, including the Ebola and Marburg viruses, cause hemorrhagic fevers with up to 90% lethality. The viral nucleocapsid is assembled by polymerization of the nucleoprotein (NP) along the viral genome, together with the viral proteins VP24 and VP35. We employed cryo-electron tomography of cells transfected with viral proteins and infected with model Ebola virus to illuminate assembly intermediates, as well as a 9 Å map of the complete intracellular assembly. This structure reveals a previously unresolved third and outer layer of NP complexed with VP35. The intrinsically disordered region, together with the C-terminal domain of this outer layer of NP, provides the constant width between intracellular nucleocapsid bundles and likely functions as a flexible tether to the viral matrix protein in the virion. A comparison of intracellular nucleocapsids with prior in-virion nucleocapsid structures reveals that the nucleocapsid further condenses vertically in the virion. The interfaces responsible for nucleocapsid assembly are highly conserved and offer targets for broadly effective antivirals.","journal":"Cell","year":2024,"id":421431,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9575,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":618415,"name":"Dawid Zyla","orcid":"0000-0001-8471-469X","position":1,"is_corresponding":false},{"id":618421,"name":"Diptiben Parekh","orcid":"0000-0002-1972-9462","position":2,"is_corresponding":false},{"id":1214207,"name":"Connor Hong","orcid":null,"position":3,"is_corresponding":false},{"id":685893,"name":"Ying Jones","orcid":null,"position":4,"is_corresponding":false},{"id":90279,"name":"Sharon L. Schendel","orcid":"0000-0002-5062-7261","position":5,"is_corresponding":false},{"id":915669,"name":"William Wan","orcid":"0000-0003-2497-3010","position":6,"is_corresponding":false},{"id":227586,"name":"Guillaume A. Castillon","orcid":"0000-0003-0657-1683","position":7,"is_corresponding":false},{"id":104945,"name":"Erica Ollmann Saphire","orcid":"0000-0002-1206-7451","position":8,"is_corresponding":false},{"id":239985,"name":"Reika Watanabe","orcid":"0000-0002-7427-7702","position":0,"is_corresponding":true}],"reference_count":83,"raw_metadata":null,"created_at":"2026-07-19T01:57:36.298171Z","pmid":"39293445","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}