{"doi":"10.1016/j.cell.2021.05.033","title":"Coupling of N7-methyltransferase and 3′-5′ exoribonuclease with SARS-CoV-2 polymerase reveals mechanisms for capping and proofreading","abstract":null,"journal":"Cell","year":2021,"id":636007,"datarank":0.7565137675378871,"base_score":5.043425116919247,"endowment":5.043425116919247,"self_citation_contribution":0.7565137675378871,"citation_network_contribution":0.0,"self_endowment_contribution":0.7565137675378871,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":154,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1650312,"name":"Yunxiang Yang","orcid":null,"position":1,"is_corresponding":false},{"id":264061,"name":"Mingyu Li","orcid":"0000-0003-1217-2929","position":2,"is_corresponding":false},{"id":832049,"name":"Ying Zhang","orcid":"0000-0003-2194-7116","position":3,"is_corresponding":false},{"id":1650313,"name":"Litao Zheng","orcid":null,"position":4,"is_corresponding":false},{"id":1650314,"name":"Ji Ge","orcid":null,"position":5,"is_corresponding":false},{"id":1650315,"name":"Yucen C. Huang","orcid":null,"position":6,"is_corresponding":false},{"id":231868,"name":"Zhenyu Liu","orcid":"0000-0003-2893-5396","position":7,"is_corresponding":false},{"id":1054302,"name":"Tao Wang","orcid":"0000-0003-3322-7554","position":8,"is_corresponding":false},{"id":994218,"name":"Shan Gao","orcid":"0000-0002-0281-4492","position":9,"is_corresponding":false},{"id":516215,"name":"Ran Zhang","orcid":"0000-0001-8353-5374","position":10,"is_corresponding":false},{"id":1650316,"name":"Yuanyun Y. Huang","orcid":null,"position":11,"is_corresponding":false},{"id":1225997,"name":"Luke W. Guddat","orcid":"0000-0002-8204-8408","position":12,"is_corresponding":false},{"id":774628,"name":"Yan Gao","orcid":"0000-0003-3698-1413","position":13,"is_corresponding":false},{"id":941940,"name":"Zihe Rao","orcid":"0000-0001-9866-2384","position":14,"is_corresponding":false},{"id":1345276,"name":"Zhiyong Lou","orcid":"0000-0003-2728-881X","position":15,"is_corresponding":false},{"id":1518834,"name":"Liming Yan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Coupling of N7-methyltransferase and 3′-5′ exoribonuclease with SARS-CoV-2 polymerase reveals mechanisms for capping and proofreading","abstract":"The capping of mRNA and the proofreading play essential roles in SARS-CoV-2 replication and transcription. Here, we present the cryo-EM structure of the SARS-CoV-2 replication-transcription complex (RTC) in a form identified as Cap(0)-RTC, which couples a co-transcriptional capping complex (CCC) composed of nsp12 NiRAN, nsp9, the bifunctional nsp14 possessing an N-terminal exoribonuclease (ExoN) and a C-terminal N7-methyltransferase (N7-MTase), and nsp10 as a cofactor of nsp14. Nsp9 and nsp12 NiRAN recruit nsp10/nsp14 into the Cap(0)-RTC, forming the N7-CCC to yield cap(0) (<sup>7Me</sup>GpppA) at 5' end of pre-mRNA. A dimeric form of Cap(0)-RTC observed by cryo-EM suggests an in trans backtracking mechanism for nsp14 ExoN to facilitate proofreading of the RNA in concert with polymerase nsp12. These results not only provide a structural basis for understanding co-transcriptional modification of SARS-CoV-2 mRNA but also shed light on how replication fidelity in SARS-CoV-2 is maintained.","is_dataset_classified":null,"base_score":5.043425116919247,"endowment":5.043425116919247,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34143953","pmcid":"PMC8142856","openalex_id":"https://openalex.org/W3165788001","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"","title":null}],"total_grants":1,"fwci":9.5551,"citation_percentile":0.99101605,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2021,"count":20},{"year":2022,"count":54},{"year":2023,"count":33},{"year":2024,"count":25},{"year":2025,"count":16},{"year":2026,"count":5}],"oa_status":"bronze","license":"http://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"http://www.cell.com/article/S0092867421006632/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S0092867421006632/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0092867421006632?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0092867421006632?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.cell.2021.05.033","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34143953","host_type":"repository"},{"url":"https://resolver.caltech.edu/CaltechAUTHORS:20210701-155032256","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8142856","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8142856","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8142856?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Enzyme Structure and Function","DNA Repair Mechanisms","Cancer Research and Treatments","Amino Acid Sequence","COVID-19","Coronavirus RNA-Dependent RNA Polymerase","Exoribonucleases","Humans","Methyltransferases","RNA, Messenger","RNA, Viral","SARS-CoV-2","Sequence Alignment","Transcription, Genetic","Virus Replication"],"mesh_terms":["COVID-19","SARS-CoV-2","Coronavirus RNA-Dependent RNA Polymerase","Amino Acid Sequence","Exoribonucleases","Humans","Methyltransferases","RNA, Messenger","RNA, Viral","Transcription, Genetic","Virus Replication","Sequence Alignment"],"keywords":["Proofreading","Biology","Exoribonuclease","Genetics","Polymerase","Coupling (piping)","Cell biology","DNA","Gene","RNA","Cryo-em","Mrna Capping","Sars-cov-2","Replication-Transcription Complex"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T15:59:14.821897Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}