{"doi":"10.1016/j.cell.2021.04.032","title":"B cell genomics behind cross-neutralization of SARS-CoV-2 variants and SARS-CoV","abstract":"Monoclonal antibodies (mAbs) are a focus in vaccine and therapeutic design to counteract severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants. Here, we combined B cell sorting with single-cell VDJ and RNA sequencing (RNA-seq) and mAb structures to characterize B cell responses against SARS-CoV-2. We show that the SARS-CoV-2-specific B cell repertoire consists of transcriptionally distinct B cell populations with cells producing potently neutralizing antibodies (nAbs) localized in two clusters that resemble memory and activated B cells. Cryo-electron microscopy structures of selected nAbs from these two clusters complexed with SARS-CoV-2 spike trimers show recognition of various receptor-binding domain (RBD) epitopes. One of these mAbs, BG10-19, locks the spike trimer in a closed conformation to potently neutralize SARS-CoV-2, the recently arising mutants B.1.1.7 and B.1.351, and SARS-CoV and cross-reacts with heterologous RBDs. Together, our results characterize transcriptional differences among SARS-CoV-2-specific B cells and uncover cross-neutralizing Ab targets that will inform immunogen and therapeutic design against coronaviruses.","journal":"Cell","year":2021,"id":1406,"datarank":0.6782682865573562,"base_score":4.5217885770490405,"endowment":4.5217885770490405,"self_citation_contribution":0.6782682865573562,"citation_network_contribution":0.0,"self_endowment_contribution":0.6782682865573562,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":91,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0365,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-06-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":16968,"name":"Christopher O. Barnes","orcid":"0000-0003-2754-5951","position":1,"is_corresponding":false},{"id":6112,"name":"Basak Eraslan","orcid":"0000-0003-3698-8279","position":2,"is_corresponding":false},{"id":16969,"name":"Andrew Hudak","orcid":"0000-0002-0241-2290","position":3,"is_corresponding":false},{"id":16970,"name":"Jennifer R. Keeffe","orcid":"0000-0002-5317-6398","position":4,"is_corresponding":false},{"id":16971,"name":"Lisa A. Cosimi","orcid":"0000-0002-5269-1881","position":5,"is_corresponding":false},{"id":16973,"name":"Frauke Muecksch","orcid":"0000-0002-0132-5101","position":7,"is_corresponding":false},{"id":16974,"name":"Yiska Weisblum","orcid":"0000-0002-9249-1745","position":8,"is_corresponding":false},{"id":16975,"name":"Shuting Zhang","orcid":"0000-0001-9140-8893","position":9,"is_corresponding":false},{"id":7838,"name":"Toni Marie Delorey","orcid":"0000-0001-6614-3803","position":10,"is_corresponding":false},{"id":16976,"name":"Ann E. Woolley","orcid":"0000-0002-2810-1618","position":11,"is_corresponding":false},{"id":16977,"name":"Fadi Ghantous","orcid":"0000-0002-6772-6901","position":12,"is_corresponding":false},{"id":16979,"name":"Devan R. Phillips","orcid":"0000-0002-9240-4912","position":14,"is_corresponding":false},{"id":16981,"name":"Kathryn E. Huey-Tubman","orcid":null,"position":16,"is_corresponding":false},{"id":16982,"name":"Alexander A. Cohen","orcid":"0000-0002-2818-656X","position":17,"is_corresponding":false},{"id":16983,"name":"Priyanthi N.P. Gnanapragasam","orcid":null,"position":18,"is_corresponding":false},{"id":16984,"name":"Kara M. Rzasa","orcid":"0000-0002-5753-7517","position":19,"is_corresponding":false},{"id":16985,"name":"Theodora Hatziioanno","orcid":null,"position":20,"is_corresponding":false},{"id":16986,"name":"Michael A. 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Scheid","orcid":"0000-0001-5524-6225","position":0,"is_corresponding":true}],"reference_count":114,"raw_metadata":null,"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}