{"doi":"10.1016/j.cell.2020.09.056","title":"Chromatin Potential Identified by Shared Single-Cell Profiling of RNA and Chromatin","abstract":"Cell differentiation and function are regulated across multiple layers of gene regulation, including modulation of gene expression by changes in chromatin accessibility. However, differentiation is an asynchronous process precluding a temporal understanding of regulatory events leading to cell fate commitment. Here we developed simultaneous high-throughput ATAC and RNA expression with sequencing (SHARE-seq), a highly scalable approach for measurement of chromatin accessibility and gene expression in the same single cell, applicable to different tissues. Using 34,774 joint profiles from mouse skin, we develop a computational strategy to identify cis-regulatory interactions and define domains of regulatory chromatin (DORCs) that significantly overlap with super-enhancers. During lineage commitment, chromatin accessibility at DORCs precedes gene expression, suggesting that changes in chromatin accessibility may prime cells for lineage commitment. We computationally infer chromatin potential as a quantitative measure of chromatin lineage-priming and use it to predict cell fate outcomes. SHARE-seq is an extensible platform to study regulatory circuitry across diverse cells in tissues.","journal":"Cell","year":2020,"id":787,"datarank":1.0613713295967582,"base_score":7.075808863978387,"endowment":7.075808863978387,"self_citation_contribution":1.0613713295967582,"citation_network_contribution":0.0,"self_endowment_contribution":1.0613713295967582,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1182,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0811,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-11-01","fair_score":73.75,"fair_percentile":99.14248021108179,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":7638,"name":"Bing Zhang","orcid":"0000-0001-8676-2425","position":1,"is_corresponding":false},{"id":7639,"name":"Lindsay M. 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