{"doi":"10.1016/j.cell.2016.09.045","title":"Genomic Nucleosome Organization Reconstituted with Pure Proteins","abstract":"Chromatin remodelers regulate genes by organizing nucleosomes around promoters, but their individual contributions are obfuscated by the complex in vivo milieu of factor redundancy and indirect effects. Genome-wide reconstitution of promoter nucleosome organization with purified proteins resolves this problem and is therefore a critical goal. Here, we reconstitute four stages of nucleosome architecture using purified components: yeast genomic DNA, histones, sequence-specific Abf1/Reb1, and remodelers RSC, ISW2, INO80, and ISW1a. We identify direct, specific, and sufficient contributions that in vivo observations validate. First, RSC clears promoters by translating poly(dA:dT) into directional nucleosome removal. Second, partial redundancy is recapitulated where INO80 alone, or ISW2 at Abf1/Reb1sites, positions +1 nucleosomes. Third, INO80 and ISW2 each align downstream nucleosomal arrays. Fourth, ISW1a tightens the spacing to canonical repeat lengths. Such a minimal set of rules and proteins establishes core mechanisms by which promoter chromatin architecture arises through a blend of redundancy and specialization.","journal":"Cell","year":2016,"id":7525,"datarank":0.8590271621380796,"base_score":5.726847747587197,"endowment":5.726847747587197,"self_citation_contribution":0.8590271621380796,"citation_network_contribution":0.0,"self_endowment_contribution":0.8590271621380796,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":306,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0527,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2016-10-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":68356,"name":"Megha Wal","orcid":null,"position":1,"is_corresponding":false},{"id":68357,"name":"Shinya Watanabe","orcid":"0000-0001-8842-6716","position":2,"is_corresponding":false},{"id":68358,"name":"Bongsoo Park","orcid":"0000-0003-1720-9567","position":3,"is_corresponding":false},{"id":68359,"name":"Craig L. Peterson","orcid":"0000-0002-9448-555X","position":4,"is_corresponding":false},{"id":19590,"name":"B. Franklin Pugh","orcid":"0000-0001-8341-4476","position":5,"is_corresponding":false},{"id":68360,"name":"Philipp Korber","orcid":"0000-0001-7526-6549","position":6,"is_corresponding":false},{"id":68355,"name":"Nils Krietenstein","orcid":"0000-0003-2519-6305","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}