{"doi":"10.1016/j.cej.2025.171992","title":"Enhancing petroleum hydrocarbon degradation by a synthetic bacterial consortium: Insights into functional complementarity and regulatory coordination","abstract":null,"journal":"Chemical Engineering Journal","year":2026,"id":608805,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1564012,"name":"Jinyu Hou","orcid":null,"position":1,"is_corresponding":false},{"id":208696,"name":"Hao Ling","orcid":null,"position":2,"is_corresponding":false},{"id":1410211,"name":"Xiaobing Wang","orcid":"0000-0002-0907-364X","position":3,"is_corresponding":false},{"id":1564013,"name":"Yuting Liang","orcid":null,"position":4,"is_corresponding":false},{"id":1564014,"name":"Wuxing Liu","orcid":null,"position":5,"is_corresponding":false},{"id":1198854,"name":"Yongming Luo","orcid":"0000-0002-2217-3207","position":6,"is_corresponding":false},{"id":1564011,"name":"Xiongxiong Wang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Enhancing petroleum hydrocarbon degradation by a synthetic bacterial consortium: Insights into functional complementarity and regulatory coordination","abstract":"Microbial remediation technology is widely applied for treating petroleum-contaminated soil. While combining strains with complementary functions can address the limited bioavailability and compositional complexity of petroleum pollutants, empirical mixtures often lack functional stability under fluctuating environmental conditions. In this study, we systematically evaluated multiple strain combinations and identified an optimized petroleum-degrading consortium, CoA, composed of a biosurfactant-producing degrader ( Pseudomonas sp. SB) with two hydrocarbon-degrading strains ( Pseudomonas extremorientalis 10–2 and Acinetobacter halotolerans PB). The consortium CoA showed significantly enhanced petroleum hydrocarbon degradation compared to its individual members (35.53 % – 47.18 % improvement) and all other tested combinations. Additionally, CoA exhibited strong tolerance to pH fluctuations, salinity, and toxic petroleum-derived intermediates such as naphthenic acids. Genomic analysis revealed metabolic specialization and complementarity among the consortium CoA. Furthermore, CoA appears to employ a dynamic regulatory mechanism mediated by the transcriptional regulator FleQ, which balances motility and biofilm formation, coordination that facilitates efficient degradation of pollutant substrates. In soil microcosm experiments, consortium CoA achieved a total petroleum hydrocarbon removal efficiency of 59.75 % after 90 days. For the poorly bioavailable C21–C40 fraction, CoA outperformed the fertilizer-only treatment by 25.23 %. Collectively, this study provides insights into the design principles of synthetic microbial consortia for petroleum pollutant degradation and highlights the role of internal regulatory mechanisms in consortium functionality. • A functionally complementary consortium was established via positive interactions. • CoA showed markedly higher petroleum hydrocarbon degradation than individual strains. • CoA maintained strong adaptability under salinity, pH, and toxic intermediates. • Genomic analyses revealed FleQ-mediated regulation of motility and biofilm.","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":"https://openalex.org/W7115571811","authors":[],"funders":[{"funder_name":"National Key Research and Development Program of China","grant_id":"2021YFC1808901","title":null}],"total_grants":1,"fwci":1.2463,"citation_percentile":0.79166204,"influential_citations":0,"citation_trend":[{"year":2026,"count":4}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://doi.org/10.1016/j.cej.2025.171992","host_type":"journal"},{"url":"https://doi.org/10.1016/j.cej.2025.171992","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1385894725128416?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1385894725128416?httpAccept=text/plain","host_type":"publisher"}],"fields_of_study":["Microbial bioremediation and biosurfactants","Microbial Applications in Construction Materials","Pesticide and Herbicide Environmental Studies"],"mesh_terms":[],"keywords":["Bioaugmentation","Bioremediation","Microbial consortium","Petroleum","Biostimulation","Rhamnolipid","Microcosm","Pollutant","Hydrocarbon"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T22:32:36.082304Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}