{"doi":"10.1016/j.ceca.2024.102983","title":"All-optical mapping of Ca2+ transport and homeostasis in dendrites","abstract":"• All-optical system for perturbation and mapping of cellular Ca 2+ . • Maps of Ca 2+ diffusion, length constant, and equilibration time in neuronal dendrites. • Distal dendrites showed nonlinear activation of Ca 2+ efflux via the NCX1 antiporter Calcium mediates many important signals in dendrites. However, the basic transport properties of calcium in dendrites have been difficult to measure: how far and how fast does a local influx of calcium propagate? We developed an all-optical system for simultaneous targeted Ca 2+ import and Ca 2+ concentration mapping. We co-expressed a blue light-activated calcium selective channelrhodopsin, CapChR2, with a far-red calcium sensor, FR-GECO1c, in cultured rat hippocampal neurons, and used patterned optogenetic stimulation to introduce calcium into cells with user-defined patterns of space and time. We determined a mean steady-state length constant for Ca 2+ transport ϕ ∼ 5.8 μm, a half-life for return to baseline t 1/2 ∼ 1.7 s, and an effective diffusion coefficient D ∼ 20 μm 2 /s, though there were substantial differences in Ca 2+ dynamics between proximal and distal dendrites. At high Ca 2+ concentration, distal dendrites showed nonlinear activation of Ca 2+ efflux, which we pharmacologically ascribed to the NCX1 antiporter. Genetically encoded tools for all-optical mapping of Ca 2+ transport and handling provide a powerful capability for studying this important messenger.","journal":"Cell Calcium","year":2024,"id":449681,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8805,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":253698,"name":"Adam E. Cohen","orcid":"0000-0002-8699-2404","position":1,"is_corresponding":false},{"id":1115437,"name":"Rebecca Frank Hayward","orcid":"0009-0006-2132-7522","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T02:02:20.585759Z","pmid":"39662137","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}