{"doi":"10.1016/j.ceca.2007.07.009","title":"RNF24, a new TRPC interacting protein, causes the intracellular retention of TRPC","abstract":null,"journal":"Cell Calcium","year":2008,"id":683931,"datarank":0.5709993734655481,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"self_citation_contribution":0.5709993734655481,"citation_network_contribution":0.0,"self_endowment_contribution":0.5709993734655481,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":44,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":152550,"name":"Pascale K. Lepage","orcid":null,"position":1,"is_corresponding":false},{"id":152553,"name":"Simon M. Bousquet","orcid":null,"position":2,"is_corresponding":false},{"id":152556,"name":"Guylain Boulay","orcid":null,"position":3,"is_corresponding":false},{"id":152551,"name":"Marc P. Lussier","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"RNF24, a new TRPC interacting protein, causes the intracellular retention of TRPC","abstract":"TRPCs function as cation channels in non-excitable cells. The N-terminal tails of all TRPCs contain an ankyrin-like repeat domain, one of the most common protein-protein interaction motifs. Using a yeast two-hybrid screening approach, we found that RNF24, a new membrane RING-H2 protein, interacted with the ankyrin-like repeat domain of TRPC6. GST pull-down and co-immunoprecipitation assays showed that RNF24 interacted with all TRPCs. Cell surface-labelling assays showed that the expression of TRPC6 at the surface of HEK 293T cells was greatly reduced when it was transiently co-transfected with RNF24. Confocal microscopy showed that TRPC3 and TRPC6 co-localized with RNF24 in a perinuclear compartment and that RNF24 co-localized with mannosidase II, a marker of the Golgi cisternae. Using a pulse-chase approach, we showed that RNF24 did not alter the maturation process of TRPC6. Moreover, in HEK 293T cells, RNF24 did not alter carbachol-induced Ca(2+) entry via endogenous channels or TRPC6. These results indicate that RNF24 interacts with TRPCs in the Golgi apparatus and affects TRPC intracellular trafficking without affecting their activity.","is_dataset_classified":null,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17850865","pmcid":null,"openalex_id":"https://openalex.org/W2062417009","authors":[],"funders":[],"total_grants":0,"fwci":0.8502,"citation_percentile":0.70247224,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2014,"count":6},{"year":2015,"count":2},{"year":2016,"count":6},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":1},{"year":2024,"count":3},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0143416007001510?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0143416007001510?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ceca.2007.07.009","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17850865","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Ion Channels and Receptors","Endoplasmic Reticulum Stress and Disease","Amino Acid Sequence","Ankyrin Repeat","Carbachol","Carrier Proteins","Cell Line","Cell Membrane","Golgi Apparatus","Humans","Membrane Proteins","Molecular Sequence Data","TRPC Cation Channels","TRPC6 Cation Channel","Ubiquitin-Protein Ligases"],"mesh_terms":["TRPC6 Cation Channel","Amino Acid Sequence","Carbachol","Carrier Proteins","Cell Line","Cell Membrane","Golgi Apparatus","Humans","Membrane Proteins","Molecular Sequence Data","Ankyrin Repeat","Ubiquitin-Protein Ligases","TRPC Cation Channels"],"keywords":["TRPC","TRPC6","TRPC3","Cell biology","TRPV","Chemistry","Transient receptor potential channel","HEK 293 cells","Intracellular","TRPC5","Transfection","Scaffold protein","Golgi apparatus","Biophysics","Biology","Biochemistry","Endoplasmic reticulum","Signal transduction","Receptor","TRPV1"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T12:45:04.172309Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}