{"doi":"10.1016/j.cdnut.2024.102144","title":"Human Milk Composition Is Associated with Maternal Body Mass Index in a Cross-Sectional, Untargeted Metabolomics Analysis of Human Milk from Guatemalan Mothers","abstract":"BackgroundMaternal overweight/obesity has been associated with poor lactation performance including delayed lactogenesis and reduced duration. However, the effect on human milk composition is less well understood.ObjectiveWe evaluated the relationship of maternal body mass index (BMI) on the human milk metabolome among Guatemalan mothers.MethodsWe used data from 75 Guatemalan mothers who participated in the Household Air Pollution Intervention Network (HAPIN) trial. Maternal BMI was measured between 9-<20 weeks gestation. Milk samples were collected at a single time point using aseptic collection from one breast at six months postpartum and analyzed using high-resolution mass spectrometry. A cross-sectional untargeted high-resolution metabolomics (HRM) analysis was performed by coupling hydrophilic interaction liquid chromatography (HILIC) and reverse phase C18 chromatography with mass spectrometry. Metabolic features associated with maternal BMI were determined by a metabolome-wide association study (MWAS), adjusting for baseline maternal age, education, and dietary diversity, and perturbations in metabolic pathways were identified by pathway enrichment analysis.ResultsThe mean age of participants at baseline was 23.62 ± 3.81 years and mean BMI was 24.27 ± 4.22 kg/m2. Of the total metabolic features detected by HILIC column (19,199 features) and by C18 column (11,594 features), BMI was associated with 1026 HILIC and 500 C18 features. Enriched pathways represented amino acid metabolism, galactose metabolism and xenobiotic metabolic metabolism. However, no significant features were identified after adjusting for multiple comparisons using the Benjamini-Hochberg false discovery rate procedure (FDRBH<0.2).ConclusionsFindings from this untargeted MWAS indicate that maternal BMI is associated with metabolic perturbations of galactose metabolism, xenobiotic metabolism, xenobiotic metabolism by cytochrome p450 and biosynthesis of amino acid pathways. Significant metabolic pathway alterations detected in human milk were associated with energy metabolism related pathways including carbohydrate and amino acid metabolism.Clinical Trial Registry Number and WebsiteClinicalTrials.gov (NCT02944682)HAPIN Clinical trials registrationClinicaltrials.gov NCT02944682","journal":"Current Developments in Nutrition","year":2024,"id":450108,"datarank":0.2706767013430035,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0292610144778884,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0292610144778884,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":4,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9417,"is_data_producer":true,"deposit_databanks":{"ClinicalTrials.gov":["NCT02944682"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":239774,"name":"Donghai Liang","orcid":"0000-0001-7311-2298","position":1,"is_corresponding":false},{"id":1270690,"name":"Carolyn Jonas Accardi","orcid":null,"position":2,"is_corresponding":false},{"id":461385,"name":"Anaité Díaz-Artiga","orcid":"0000-0002-2508-8710","position":3,"is_corresponding":false},{"id":300624,"name":"Xin Hu","orcid":"0000-0003-2747-9085","position":4,"is_corresponding":false},{"id":687912,"name":"Erick Mollinedo","orcid":"0000-0002-7769-101X","position":5,"is_corresponding":false},{"id":371409,"name":"Usha Ramakrishnan","orcid":"0000-0003-1694-1238","position":6,"is_corresponding":false},{"id":1066868,"name":"Sami Teeny","orcid":"0009-0000-1271-2492","position":7,"is_corresponding":false},{"id":234190,"name":"ViLinh Tran","orcid":null,"position":8,"is_corresponding":false},{"id":268904,"name":"Thomas Clasen","orcid":"0000-0003-4062-5788","position":9,"is_corresponding":false},{"id":268907,"name":"Lisa M. Thompson","orcid":"0000-0002-8001-2057","position":10,"is_corresponding":false},{"id":687921,"name":"Sheela Sinharoy","orcid":"0000-0003-3077-3824","position":11,"is_corresponding":false},{"id":1270316,"name":"Kasthuri Sivalogan","orcid":"0000-0002-8500-630X","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-19T02:02:25.004119Z","pmid":"38726027","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}