{"doi":"10.1016/j.cbpb.2005.03.004","title":"A comparison of the counteracting effects of glycine betaine and TMAO on the activity of RNase A in aqueous urea solution","abstract":null,"journal":"Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology","year":2005,"id":684129,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1787250,"name":"J.J. Bedford","orcid":null,"position":1,"is_corresponding":false},{"id":1787251,"name":"R.A.J. Smith","orcid":null,"position":2,"is_corresponding":false},{"id":1787252,"name":"J.P. Leader","orcid":null,"position":3,"is_corresponding":false},{"id":1787249,"name":"L.M. Samuelsson","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A comparison of the counteracting effects of glycine betaine and TMAO on the activity of RNase A in aqueous urea solution","abstract":"Trimethylamine-N-oxide (TMAO) and glycine betaine are counteracting osmolytes found in cellular systems under osmotic stress, often in association with high urea concentrations. TMAO is a characteristic component of cartilaginous fish and marine molluscs, while glycine betaine is more widely distributed, occurring in plants, bacteria and the mammalian kidney. As part of a project to explain and understand the action of these methylamines, the RNase A-catalysed degradation of polyuridylic acid in the presence of urea and various osmolytes (0-1.0 M) was studied using (31)P Nuclear Magnetic Resonance spectroscopy. The decrease in reaction rate induced by urea could be fully recovered with 1 molar equivalent of trimethylamine-N-oxide or 1.4 molar equivalents of glycine betaine. These results indicate that the modification of RNase A activity induced by urea is not associated with gross irreversible structural changes and that both glycine betaine and trimethylamine-N-oxide have kinetically detectable counteracting effects.","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15886035","pmcid":null,"openalex_id":"https://openalex.org/W2044411855","authors":[],"funders":[],"total_grants":0,"fwci":0.1695,"citation_percentile":0.51102057,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2014,"count":2},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1095643305000723?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1095643305000723?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.cbpb.2005.03.004","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15886035","host_type":"repository"}],"fields_of_study":["Mass Spectrometry Techniques and Applications","Metabolomics and Mass Spectrometry Studies","Environmental Chemistry and Analysis","Animals","Betaine","Catalysis","Cattle","Magnetic Resonance Spectroscopy","Methylamines","Molecular Structure","RNA","Ribonuclease, Pancreatic","Solutions","Urea"],"mesh_terms":["Animals","Betaine","Catalysis","Cattle","Methylamines","Magnetic Resonance Spectroscopy","Ribonuclease, Pancreatic","RNA","Solutions","Urea","Molecular Structure"],"keywords":["Betaine","Osmolyte","Trimethylamine N-oxide","Trimethylamine","Glycine","Urea","Chemistry","Methylamines","Aqueous solution","Biochemistry","Nuclear chemistry","Organic chemistry","Methylamine","Amino acid"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T13:22:38.183724Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}