{"doi":"10.1016/j.bpj.2009.08.003","title":"Two-Path Impedance Spectroscopy for Measuring Paracellular and Transcellular Epithelial Resistance","abstract":null,"journal":"Biophysical Journal","year":2009,"id":625546,"datarank":0.6995158641168101,"base_score":4.663439094112067,"endowment":4.663439094112067,"self_citation_contribution":0.6995158641168101,"citation_network_contribution":0.0,"self_endowment_contribution":0.6995158641168101,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":105,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":204712,"name":"Michael Fromm","orcid":null,"position":1,"is_corresponding":false},{"id":204708,"name":"Dorothee Günzel","orcid":"0000-0002-7998-7164","position":2,"is_corresponding":false},{"id":207480,"name":"Susanne M. Krug","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Two-Path Impedance Spectroscopy for Measuring Paracellular and Transcellular Epithelial Resistance","abstract":"Solutes are transported across epithelial cell layers via transcellular and paracellular pathways. The transcellular pathway leads across the apical and basolateral cell membrane, whereas the paracellular pathway is directed through the tight junction. Tight junction proteins (claudins, occludin, tricellulin) can not only form barriers but also paracellular channels that are--in concert with membrane channels and transporters--regulated in a wide range in health and disease states. Thus, it is desirable to determine para- and transcellular resistance (R(para), R(trans)) separately. This cannot be achieved by conventional transepithelial resistance (TER) measurements. We present an impedance spectroscopic approach that is optimized for differentiation between these two pathways. The method is based on a transepithelial impedance measurement in specialized Ussing chambers, combined with a Ca(2+)-dependent modulation of R(para) through EGTA and flux measurements of a nonradioactive paracellular marker, fluorescein. The prerequisites are a paracellular marker that varies in parallel to 1/R(para), an experimental regime that alters R(para) without affecting R(trans), and exact knowledge of the resistance of subepithelial components. The underlying prerequisites and the applicability as a routine method were verified on cell lines of different tightness including HT-29/B6 colon cells and Madin-Darby canine kidney tubule cells C7 and C11.","is_dataset_classified":null,"base_score":4.663439094112067,"endowment":4.663439094112067,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19843452","pmcid":"PMC2764069","openalex_id":"https://openalex.org/W2091987168","authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"DFG GU447/11-1","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"FOR 721","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"}],"total_grants":3,"fwci":6.7901,"citation_percentile":0.96820259,"influential_citations":0,"citation_trend":[{"year":2012,"count":16},{"year":2013,"count":7},{"year":2014,"count":9},{"year":2015,"count":4},{"year":2016,"count":7},{"year":2017,"count":9},{"year":2018,"count":2},{"year":2019,"count":3},{"year":2020,"count":11},{"year":2021,"count":8},{"year":2022,"count":2},{"year":2023,"count":3},{"year":2024,"count":4},{"year":2025,"count":6}],"oa_status":"bronze","license":"Elsevier Non-Commercial","oa_locations":[{"url":"http://www.cell.com/article/S0006349509013502/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S0006349509013502/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006349509013502?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006349509013502?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bpj.2009.08.003","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19843452","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2764069","host_type":"repository"},{"url":"http://dx.doi.org/10.1016/j.bpj.2009.08.003","host_type":""},{"url":"https://dx.doi.org/10.1016/j.bpj.2009.08.003","host_type":""}],"fields_of_study":["Barrier Structure and Function Studies","Connexins and lens biology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Calcium","Cell Communication","Cell Line","Cell Line, Tumor","Cell Membrane","Chelating Agents","Colforsin","Dogs","Egtazic Acid","Electric Impedance","Epithelium","Fluorescein","Humans","Signal Transduction","Spectrum Analysis","Tight Junctions"],"mesh_terms":["Animals","Calcium","Cell Communication","Cell Line","Cell Membrane","Chelating Agents","Dogs","Egtazic Acid","Epithelium","Colforsin","Humans","Spectrum Analysis","Signal Transduction","Electric Impedance","Tight Junctions","Fluorescein","Cell Line, Tumor"],"keywords":["Paracellular transport","Transcellular","Claudin","Tight junction","Occludin","Chemistry","Biophysics","Cell biology","Ussing chamber","Epithelium","Membrane","Biology","In vitro","Biochemistry","Permeability (electromagnetism)","Spectrum Analysis","Cell Membrane","Colforsin","Cell Communication","Cell Line","Tight Junctions","Dogs","Cell Line, Tumor","Electric Impedance","Animals","Humans","Calcium","Fluorescein","Egtazic Acid","Chelating Agents","Signal Transduction"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T07:03:52.399663Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}