{"doi":"10.1016/j.biosx.2025.100664","title":"Hydrogel-based multiplexed high-risk human papillomavirus DNA/RNA detection for cervical cancer screening","abstract":"Rapid triaging of high-risk human papillomavirus (HPV) infections, especially in resource-limited regions, can reduce cervical cancer deaths by identifying high-risk patients and minimizing pathology bottlenecks and overtreatment. Here, we developed a simple, rapid method for parallel detection of HPV DNA and RNA subtypes associated with a high risk of developing cervical cancer. Specifically, we applied loop-mediated isothermal nucleic acid amplification (LAMP) in a hydrogel-based platform (LAMPGel) for HPV DNA or RNA detection using distinct hydrogel chambers within a single chip. The LAMPGel platform locally restricts single HPV gDNA or mRNA strands with a LAMP cocktail, which is then distributed within a thin hydrogel to locally form single fluorescent spots after a short isothermal incubation of < 30 min. Detecting and counting these spots identifies HPV infection, viral load, and, more importantly, RNA expression as a predictive marker for cervical cancer development. LAMPGel is a sensitive, specific, easy-to-use, and rapid method for a point-of-care (POC) platform for potential applications in low- and middle-income countries once the technology is further validated with clinical samples in those settings. Furthermore, it could find broader applications in POC nucleic acid assays. • Rapid, point-of-care HPV DNA/RNA detection using isothermal amplification in hydrogels • Hydrogel confines fluorescence signals read as individual dots • Simple counting of the number of fluorescence dots defines HPV infection and subtyping • HPV DNA/RNA multiplexing is designed to identify patients at higher risk of developing cervical cancer","journal":"Biosensors and Bioelectronics X","year":2025,"id":571151,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1208275,"name":"Jae‐Sang Hong","orcid":"0000-0002-7664-6639","position":1,"is_corresponding":false},{"id":1016890,"name":"Joshua D. Spitzberg","orcid":"0000-0002-7937-9273","position":2,"is_corresponding":false},{"id":23522,"name":"Ralph Weissleder","orcid":"0000-0003-0828-4143","position":3,"is_corresponding":false},{"id":789066,"name":"Cesar M. Castro","orcid":"0000-0002-1159-5658","position":4,"is_corresponding":false},{"id":287819,"name":"Hyungsoon Im","orcid":"0000-0002-0626-1346","position":5,"is_corresponding":false},{"id":666185,"name":"Morteza Azizi","orcid":"0009-0008-8893-2035","position":0,"is_corresponding":true}],"reference_count":14,"raw_metadata":null,"created_at":"2026-07-19T02:57:15.755535Z","pmid":"42273395","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}