{"doi":"10.1016/j.bios.2021.113901","title":"Development of a POCT type insulin sensor employing anti-insulin single chain variable fragment based on faradaic electrochemical impedance spectroscopy under single frequency measurement","abstract":null,"journal":"Biosensors and Bioelectronics","year":2022,"id":631815,"datarank":1.266233313241163,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":0.7287054724727463,"self_endowment_contribution":0.5375278407684165,"citer_contribution":0.7287054724727463,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":29,"citers_with_citation_signal":23,"citers_with_endowment":23,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1637490,"name":"Rinko Fujita","orcid":null,"position":1,"is_corresponding":false},{"id":1062006,"name":"Jinhee Lee","orcid":"0000-0001-9895-6104","position":2,"is_corresponding":false},{"id":226587,"name":"Ellie Wilson","orcid":"0000-0003-2968-0268","position":3,"is_corresponding":false},{"id":1637491,"name":"Kohei Ito","orcid":null,"position":4,"is_corresponding":false},{"id":1637492,"name":"Ryutaro Asano","orcid":null,"position":5,"is_corresponding":false},{"id":112386,"name":"Kazunori Ikebukuro","orcid":null,"position":6,"is_corresponding":false},{"id":1637495,"name":"Jeffrey LaBelle","orcid":null,"position":7,"is_corresponding":false},{"id":841331,"name":"Koji Sode","orcid":"0000-0002-9833-2091","position":8,"is_corresponding":false},{"id":1637489,"name":"Mukund Khanwalker","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Development of a POCT type insulin sensor employing anti-insulin single chain variable fragment based on faradaic electrochemical impedance spectroscopy under single frequency measurement","abstract":"To improve glycemic control managed through insulin administration, recent studies have focused on developing hand-held point-of-care testing (POCT) electrochemical biosensors for insulin measurement. Amongst them, anti-insulin IgG-based sensors show promise in detecting insulin with high specificity and sensitivity. However, fabrication of electrochemical sensors with IgG antibodies can prove challenging because of their larger molecular size. To overcome these limitations, this study focuses on utilizing the anti-insulin single chain variable fragment (scFv) as a biosensing molecule with single-frequency faradaic electrochemical impedance spectroscopy (EIS). By comparing two different immobilization methods, covalent conjugation via succinimidyl ester and non-covalent poly-histidine chelation, we demonstrated effective modification of the electrode surface with anti-insulin scFv, while retaining its specific recognition toward insulin. Sensor performance was confirmed via the concentration-dependent faradaic electrochemical impedance change using potassium ferricyanide as a redox probe. The optimal frequency for measurement was determined to be the peak slope of the calculated impedance correlation with respect to frequency. Based on the identified optimized frequency, we performed single-frequency measurement of insulin within a concentration range of 10 pM-100 nM. This study can aid in developing a future point-of-care sensor which rapidly and sensitively measures insulin across a dynamic range of physiological concentrations, with label-free detection.","is_dataset_classified":null,"base_score":3.58351893845611,"endowment":3.58351893845611,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34968857","pmcid":null,"openalex_id":"https://openalex.org/W4200276030","authors":[],"funders":[],"total_grants":0,"fwci":3.3064,"citation_percentile":0.9335472,"influential_citations":0,"citation_trend":[{"year":2022,"count":7},{"year":2023,"count":7},{"year":2024,"count":11},{"year":2025,"count":7},{"year":2026,"count":3}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0956566321009386?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0956566321009386?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bios.2021.113901","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34968857","host_type":"repository"}],"fields_of_study":["Analytical Chemistry and Sensors","Electrochemical sensors and biosensors","Microfluidic and Capillary Electrophoresis Applications","Biosensing Techniques","Dielectric Spectroscopy","Electrochemical Techniques","Electrodes","Insulin","Point-of-Care Testing","Single-Chain Antibodies"],"mesh_terms":["Point-of-Care Testing","Electrodes","Insulin","Biosensing Techniques","Electrochemical Techniques","Single-Chain Antibodies","Dielectric Spectroscopy"],"keywords":["Dielectric spectroscopy","Biosensor","Ferricyanide","Insulin","Cyclic voltammetry","Materials science","Electrical impedance","Analytical Chemistry (journal)","Chemistry","Covalent bond","Electrochemistry","Electrode","Chromatography","Nanotechnology","Biochemistry","Medicine","Organic chemistry","Single Chain Variable Fragment (Scfv)","Faradaic Electrochemical Impedance Spectroscopy","Point-of-care Testing (Poct) Sensor","Single Frequency Measurement"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T00:47:19.014326Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}