{"doi":"10.1016/j.bioelechem.2017.06.009","title":"Development of screen-printed electrode based immunosensor for the detection of HER2 antigen in human serum samples","abstract":null,"journal":"Bioelectrochemistry","year":2017,"id":649579,"datarank":0.6166310796259968,"base_score":4.110873864173311,"endowment":4.110873864173311,"self_citation_contribution":0.6166310796259968,"citation_network_contribution":0.0,"self_endowment_contribution":0.6166310796259968,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":60,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1693445,"name":"Keya Layek","orcid":null,"position":1,"is_corresponding":false},{"id":1693447,"name":"Runu Mukherjee","orcid":null,"position":2,"is_corresponding":false},{"id":1693448,"name":"Kalyan Kumar Mistry","orcid":null,"position":3,"is_corresponding":false},{"id":1693450,"name":"Monidipa Ghosh","orcid":null,"position":4,"is_corresponding":false},{"id":1693444,"name":"Sagarika Deepthy Tallapragada","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Development of screen-printed electrode based immunosensor for the detection of HER2 antigen in human serum samples","abstract":"In this study, an immunosensor based on screen-printed electrode (SPE) has been developed for the detection of Human Epidermal Growth Factor Receptor-2 (HER2) antigen. The SPEs were fabricated and a sandwich enzyme linked immunosorbent assay (ELISA) format was followed for the construction of the immunosensor. The capture antibody (mouse anti-human ErbB2) was coated onto the electrode surface without any prior surface modification, followed by the addition of recombinant human HER2 antigen. Biotinylated goat anti-human ErbB2 was used as the detection antibody which was linked to streptavidin conjugated horseradish peroxidase (HRP). 3,3',5,5'-tetramethylbenzidine (TMB) was used as the substrate. The redox reaction was measured using cyclic voltammetry at scan rate of 50mV/s for the quantification of the antigen in solution. The biotin-avidin chemistry enabled the accurate detection of the antigen in nanogram levels. The amperometric signal obtained increased linearly with increase in the HER2 concentration and two-fold linear range was obtained between 5ng/ml-20ng/ml and 20-200ng/ml respectively. The limit of detection (LOD) and the limit of quantification (LOQ) of this immunosensor were found to be 4ng/ml and 5ng/ml respectively. The detection of HER2 in the serum samples of invasive and non-invasive breast cancer patients has been realized.","is_dataset_classified":null,"base_score":4.110873864173311,"endowment":4.110873864173311,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28692846","pmcid":null,"openalex_id":"https://openalex.org/W2726025845","authors":[],"funders":[{"funder_name":"CSIR","grant_id":"ESC0112/RP/II/T4.2","title":null}],"total_grants":1,"fwci":4.6365,"citation_percentile":0.95356074,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2018,"count":5},{"year":2019,"count":9},{"year":2020,"count":9},{"year":2021,"count":11},{"year":2022,"count":7},{"year":2023,"count":3},{"year":2024,"count":9},{"year":2025,"count":4},{"year":2026,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1567539416302584?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1567539416302584?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bioelechem.2017.06.009","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28692846","host_type":"repository"}],"fields_of_study":["Monoclonal and Polyclonal Antibodies Research","Advanced Biosensing Techniques and Applications","Nanofabrication and Lithography Techniques"],"mesh_terms":["Benzidines","Blood Chemical Analysis","Electrochemistry","Electrodes","Enzyme-Linked Immunosorbent Assay","Horseradish Peroxidase","Humans","Printing","Biosensing Techniques","Erb-b2 Receptor Tyrosine Kinases","Receptor, ErbB-2","Antibodies, Immobilized","Limit of Detection"],"keywords":["Electrode","Antigen","Chromatography","Chemistry","Biology","Immunology","Cyclic Voltammetry","Immunosensor","Screen Printed Electrode","Enzyme Linked Immunosorbent Assay (Elisa)","Human Epidermal Growth Factor Receptor-2 (Her2) Antigen"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T03:57:42.205140Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}