{"doi":"10.1016/j.bbrep.2025.102255","title":"Circadian and photoperiodic control of melatonin in Drosophila ananassae","abstract":"Melatonin is a widely conserved indoleamine with well-established roles in circadian timekeeping, sleep regulation, and physiological coordination across taxa. In Drosophila melanogaster , early studies reported daily melatonin rhythms, but little is known about melatonin dynamics in other Drosophila species. Here, we measured melatonin secretion in >14,000 Drosophila ananassae across 12 circadian timepoints under four simulated photoperiods: LD 8:16 (short day), LD 12:12 (equatorial), LD 16:8 (long day), and LD 20:4 (arctic summer). Using LC-MS/MS detection in pooled whole-fly samples, we identified robust, bimodal melatonin rhythms across all conditions, with conserved peaks at approximately ZT7 and ZT13 irrespective of daylength. Melatonin levels were significantly elevated under the short-day photoperiod compared to all others ( p < 0.0005), indicating photoperiod-sensitive amplitude modulation. These findings confirm the presence of circadian melatonin rhythms in Drosophila , replicate a previously reported early-day peak observed in D. melanogaster , and reveal a second rhythm component not described in earlier studies. The data suggest that melatonin secretion in Drosophila is governed by an endogenous clock and phase-locked to lights-on, even in the absence of nighttime darkness. This work refines our understanding of melatonin rhythmicity in insects and underscores its potential role as a conserved circadian output across species.","journal":"Biochemistry and Biophysics Reports","year":2025,"id":574683,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9393,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":367195,"name":"Justin M. Snider","orcid":"0000-0003-4102-698X","position":1,"is_corresponding":false},{"id":1482738,"name":"Hannah Doskicz","orcid":null,"position":2,"is_corresponding":false},{"id":240121,"name":"Michael A. Grandner","orcid":"0000-0002-4626-754X","position":3,"is_corresponding":false},{"id":478799,"name":"Fabian‐Xosé Fernandez","orcid":"0000-0002-4288-5383","position":4,"is_corresponding":false},{"id":1017029,"name":"Hannah K. Dollish","orcid":"0000-0002-5846-4241","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T02:57:44.572630Z","pmid":"41542173","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}