{"doi":"10.1016/j.bbrc.2023.04.108","title":"FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2023,"id":617946,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1590240,"name":"Larisa A. Varfolomeeva","orcid":"0000-0001-8150-0522","position":1,"is_corresponding":false},{"id":311259,"name":"Anna V. Vlaskina","orcid":"0000-0002-2573-3898","position":2,"is_corresponding":false},{"id":1593810,"name":"Yulia K. Agapova","orcid":null,"position":3,"is_corresponding":false},{"id":311257,"name":"A.Y. Nikolaeva","orcid":"0000-0003-4255-5170","position":4,"is_corresponding":false},{"id":11616,"name":"Kiryl D. Piatkevich","orcid":"0000-0002-7777-9468","position":5,"is_corresponding":false},{"id":1593811,"name":"Maxim V. Patrushev","orcid":null,"position":6,"is_corresponding":false},{"id":311261,"name":"Konstantin M. Boyko","orcid":"0000-0001-8229-189X","position":7,"is_corresponding":false},{"id":311266,"name":"Fedor V. Subach","orcid":"0000-0003-2720-7821","position":8,"is_corresponding":false},{"id":312978,"name":"Oksana M. Subach","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein","abstract":"Neurobiologists widely use green genetically encoded calcium indicators (GECIs) for visualization of neuronal activity. Among them, ratiometric GECIs allow imaging of both active and non-active neuronal populations. However, they are not popular, since their properties are inferior to intensiometric GCaMP series of GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. However, the dynamic range and sensitivity of its large Stock's shift green (LSS-Green) form is significantly lower than its Green form and its molecular design is not optimal. To address these drawbacks, we engineered a ratiometric green calcium indicator, called FNCaMP, which is based on bright mNeonGreen protein and calmodulin from A. niger and has optimal NTnC-like design. We compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed X-ray structure of the FNCaMP indicator.","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37163937","pmcid":null,"openalex_id":"https://openalex.org/W4367301383","authors":[],"funders":[{"funder_name":"Russian Science Foundation","grant_id":"21-74-20135","title":null},{"funder_name":"FSBSI Directorate of State Scientific and Technical Programs","grant_id":"075-15-2019-1659","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"32050410298","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"32171093","title":null},{"funder_name":"Ministry of Education and Science of the Russian Federation","grant_id":"","title":null},{"funder_name":"Westlake University","grant_id":"","title":null},{"funder_name":"National Research Center "Kurchatov Institute"","grant_id":"","title":null}],"total_grants":7,"fwci":1.9669,"citation_percentile":0.86825928,"influential_citations":0,"citation_trend":[{"year":2023,"count":2},{"year":2024,"count":3},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X23005454?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X23005454?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bbrc.2023.04.108","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37163937","host_type":"repository"}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Cell Image Analysis Techniques","Genomics and Chromatin Dynamics","Animals","Green Fluorescent Proteins","Calcium","Calmodulin","Neurons","Calcium Signaling","Mammals"],"mesh_terms":["Animals","Calcium","Calmodulin","Mammals","Neurons","Calcium Signaling","Green Fluorescent Proteins"],"keywords":["Calmodulin","Calcium","Chemistry","In vitro","Calcium imaging","Biophysics","Premovement neuronal activity","Biochemistry","Neuroscience","Biology","Organic chemistry","Crystal structure","Chromophore","Genetically Encoded Calcium Indicators","Green Fluorescent Ratiometric Fncamp Indicator"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T03:02:40.224487Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}