{"doi":"10.1016/j.bbrc.2020.01.034","title":"MicroRNA-301a (miR-301a) is induced in hepatocellular carcinoma (HCC) and down- regulates the expression of interferon regulatory factor-1","abstract":"Hepatocellular carcinoma (HCC) tumors evade death in part by downregulating expression of the tumor suppressor gene Interferon regulatory factor-1 (IRF-1). However, the molecular mechanisms accounting for IRF-1 suppression in HCC have not been well described. In this study, we identified a novel microRNA-301a (miR-301a) binding site in the 3'-untranslated region (3'- UTR) of the human IRF-1 gene and hypothesized a functional role for miR-301a in regulating HCC growth. We show that miR-301a is markedly upregulated in primary HCC tumors and HCC cell lines, while IRF-1 is down-regulated in a post-transcriptional manner. MiR-301a regulates basal and inducible IRF-1 expression in HCC cells with an inverse relationship between miR-301a and IRF-1 expression in HCC cells. Chronic hypoxia induces miR-301a in HCC in vitro and decreases IRF-1 expression. Finally, miR-301a inhibition increases apoptosis and decreases HCC cell proliferation. These findings suggest that targeting of IRF-1 by miR-301a contributes to the molecular basis for IRF-1 downregulation in HCC and provides new insight into the regulation of HCC by miRNAs.","journal":"Biochemical and Biophysical Research Communications","year":2020,"id":71930,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9591,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":280837,"name":"Qiang Du","orcid":"0009-0003-7172-5682","position":1,"is_corresponding":false},{"id":379432,"name":"Xiao Cui","orcid":"0000-0002-2456-5634","position":2,"is_corresponding":false},{"id":379433,"name":"Peiqi Wan","orcid":"0000-0003-1951-9147","position":3,"is_corresponding":false},{"id":379434,"name":"Christof Kaltenmeier","orcid":"0000-0003-1615-7443","position":4,"is_corresponding":false},{"id":284377,"name":"Jing Luo","orcid":"0000-0002-1190-553X","position":5,"is_corresponding":false},{"id":17712,"name":"Bing Yan","orcid":"0000-0002-7970-6764","position":6,"is_corresponding":false},{"id":280835,"name":"Yihe Yan","orcid":"0000-0002-3387-2273","position":7,"is_corresponding":false},{"id":280838,"name":"David A. Geller","orcid":"0000-0003-0735-1068","position":8,"is_corresponding":false},{"id":379431,"name":"Kun Dong","orcid":"0000-0003-3030-7962","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-18T21:44:25.469842Z","pmid":"31987500","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}