{"doi":"10.1016/j.bbrc.2019.07.120","title":"Storage of human whole blood, but not isolated monocytes, preserves the distribution of monocyte subsets","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2019,"id":597928,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1531961,"name":"René Weiss","orcid":null,"position":1,"is_corresponding":false},{"id":1531962,"name":"Tanja Eichhorn","orcid":null,"position":2,"is_corresponding":false},{"id":1531963,"name":"Andreas Spittler","orcid":null,"position":3,"is_corresponding":false},{"id":1531964,"name":"Michael B. Fischer","orcid":null,"position":4,"is_corresponding":false},{"id":1531965,"name":"Viktoria Weber","orcid":null,"position":5,"is_corresponding":false},{"id":1531960,"name":"Birgit Fendl","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Storage of human whole blood, but not isolated monocytes, preserves the distribution of monocyte subsets","abstract":"Human monocytes include CD14++CD16− (classical), CD14++CD16+ (intermediate), and CD14+CD16++ (non-classical) subsets with divergent roles in immune regulation and inflammation. Since the functional characterization of monocyte subsets is most commonly performed using isolated monocytes, we investigated the influence of different monocyte isolation protocols on the relative abundance of monocyte subsets. Using flow cytometric subset characterization directly in whole blood as a reference, we found that monocyte isolation by enrichment of peripheral blood mononuclear cells and subsequent depletion of non-monocytes by magnetic labeling did not alter the distribution of monocyte subsets. Particularly, we failed to detect a loss of CD16+ subsets upon monocyte isolation, although one of the negative depletion protocols used contained an anti-CD16 antibody to label granulocytes. Overnight storage of isolated monocytes induced a significant repartition of monocyte subsets towards CD14++CD16+ intermediate monocytes, which was barely seen in stored whole blood. We identified intermediate monocytes as main binding partners of platelet-derived extracellular vesicles (EVs) and propose that residual platelets contained in isolated monocyte preparations release EVs that induce the expression of the IgG receptor FcγRIII (CD16) on monocytes.","is_dataset_classified":null,"base_score":3.091042453358316,"endowment":3.091042453358316,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31387744","pmcid":null,"openalex_id":"https://openalex.org/W2966345944","authors":[],"funders":[{"funder_name":"Christian Doppler Society (Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis)","grant_id":"","title":null},{"funder_name":"EFRE","grant_id":"","title":null},{"funder_name":"regional government of Lower Austria","grant_id":"","title":null}],"total_grants":3,"fwci":1.0624,"citation_percentile":0.76196829,"influential_citations":0,"citation_trend":[{"year":2019,"count":3},{"year":2020,"count":4},{"year":2021,"count":3},{"year":2022,"count":4},{"year":2023,"count":1},{"year":2024,"count":3},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://doi.org/10.1016/j.bbrc.2019.07.120","host_type":"journal"},{"url":"https://doi.org/10.1016/j.bbrc.2019.07.120","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X19314883?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X19314883?httpAccept=text/plain","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31387744","host_type":"repository"}],"fields_of_study":["Extracellular vesicles in disease","Cell Adhesion Molecules Research","Immune cells in cancer"],"mesh_terms":["Extracellular Vesicles","Blood Platelets","Blood Preservation","Cell Separation","Humans","Monocytes"],"keywords":["CD16","CD14","Monocyte","Immunology","Biology","Platelet","Peripheral blood mononuclear cell","Whole blood","Fc receptor","Chemistry","Flow cytometry","Immune system","In vitro","Biochemistry","CD3","CD8","Extracellular Vesicles","Monocyte Subsets","Monocyte Isolation"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T14:36:22.637641Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}