{"doi":"10.1016/j.bbrc.2017.05.033","title":"RNF8 negatively regulates NF-kappaB signaling by targeting IkappaB kinase: implications for the regulation of inflammation signaling","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2017,"id":657556,"datarank":0.7496913815035551,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":0.21216354073513857,"self_endowment_contribution":0.5375278407684165,"citer_contribution":0.21216354073513857,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":11,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":25,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1716532,"name":"Jiaoxiang Wu","orcid":null,"position":1,"is_corresponding":false},{"id":94067,"name":"Lili Liang","orcid":"0000-0002-7610-9825","position":2,"is_corresponding":false},{"id":1244357,"name":"Ruixue Xu","orcid":"0009-0003-3080-7825","position":3,"is_corresponding":false},{"id":1716531,"name":"Shijuan Gao","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"RNF8 negatively regulates NF-kappaB signaling by targeting IkappaB kinase: implications for the regulation of inflammation signaling","abstract":"Persistent or excess activation of NF-κB leads to cancer, autoimmune and inflammatory diseases. Therefore, activated NF-κB needs to be terminated after induction, which highlights the physiological significance of NF-κB-negative regulators. However, the molecular mechanisms that negatively regulate NF-κB are not well understood. Here, we report that Ring Finger Protein 8 (RNF8), an E3 ubiquitin ligase, inhibits TNFα-mediated NF-κB activation by targeting IκB kinase (IKK). Upon TNFα stimulation, RNF8 binds to the catalytic subunits of IKK complex, resulting in inhibition of IKKα/β phosphorylation and subsequent NF-κB activation. RNF8 targets the IKK complex in a manner independent of its RING domain. We further provide evidence that the silencing of RNF8 results in enhanced TNFα-induced IKK activation, and an increase expression of NF-κB-induced inflammatory cytokine IL-8. Our study identifies a previously unrecognized role for RNF8 in the negative regulation of NF-κB activation by targeting and deactivating the IKK complex.","is_dataset_classified":null,"base_score":3.58351893845611,"endowment":3.58351893845611,"datacite_reuse_total":25,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28499869","pmcid":null,"openalex_id":"https://openalex.org/W2612865795","authors":[],"funders":[{"funder_name":"Beijing Natural Science Foundation","grant_id":"7122109","title":null}],"total_grants":1,"fwci":0.2383,"citation_percentile":0.48254891,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2022,"count":28},{"year":2023,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X17308926?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X17308926?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bbrc.2017.05.033","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28499869","host_type":"repository"}],"fields_of_study":["NF-κB Signaling Pathways","interferon and immune responses","Immune Response and Inflammation"],"mesh_terms":["DNA-Binding Proteins","Humans","Inflammation","Tumor Necrosis Factor-alpha","Signal Transduction","Down-Regulation","NF-kappa B","Ubiquitin-Protein Ligases","Cell Line, Tumor","I-kappa B Kinase","HEK293 Cells"],"keywords":["IκB kinase","Ubiquitin ligase","NF-κB","Phosphorylation","Ubiquitin","Signal transduction","Kinase","Cell biology","Inflammation","NFKB1","IκBα","Cancer research","Biology","Chemistry","Transcription factor","Immunology","Biochemistry","Cell signaling","Inflammatory Response","Rnf8"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.20307478.v1","title":"Additional file 11 of A functional reference map of the RNF8 interactome in cancer","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.20307478","title":"Additional file 11 of A functional reference map of the RNF8 interactome in cancer","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.20307505.v1","title":"Additional file 15 of A functional reference map of 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