{"doi":"10.1016/j.bbrc.2007.07.149","title":"O-GlcNAc modification modulates the expression of osteocalcin via OSE2 and Runx2","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2007,"id":662731,"datarank":0.5806801516361837,"base_score":3.8712010109078907,"endowment":3.8712010109078907,"self_citation_contribution":0.5806801516361837,"citation_network_contribution":0.0,"self_endowment_contribution":0.5806801516361837,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":47,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1650697,"name":"Yun-Hee Kim","orcid":null,"position":1,"is_corresponding":false},{"id":634462,"name":"Minseok Song","orcid":"0000-0003-2932-284X","position":2,"is_corresponding":false},{"id":1730219,"name":"Sang Hee An","orcid":null,"position":3,"is_corresponding":false},{"id":1730220,"name":"Ha-Young Byun","orcid":null,"position":4,"is_corresponding":false},{"id":1730221,"name":"Kyun Heo","orcid":null,"position":5,"is_corresponding":false},{"id":1730222,"name":"Seyoung Lim","orcid":null,"position":6,"is_corresponding":false},{"id":1730223,"name":"Young-Seok Oh","orcid":null,"position":7,"is_corresponding":false},{"id":918411,"name":"Sung Ho Ryu","orcid":"0000-0003-0913-3048","position":8,"is_corresponding":false},{"id":1566425,"name":"Pann-Ghill Suh","orcid":null,"position":9,"is_corresponding":false},{"id":390084,"name":"Sun-Hee Kim","orcid":"0000-0003-2582-0841","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"O-GlcNAc modification modulates the expression of osteocalcin via OSE2 and Runx2","abstract":"O-Linked beta-N-acetylglucosamine (O-GlcNAc) modification, a reversible post-translational modification, has been implicated in the regulation of protein stability, subcellular localization of proteins and protein-protein interaction. Here, we demonstrate that O-GlcNAc modification regulates the expression of osteocalcin, an osteoblast-specific marker, via Runx2 transcriptional activity in osteoblastic differentiation. Protein-associated O-GlcNAc was increased during osteoblastic differentiation in MC3T3-E1 preosteoblasts. In addition, PUGNAc, an inhibitor of O-GlcNAcase, potentiated the expression of osteocalcin caused by ascorbic acid, parathyroid hormone (PTH) and forskolin. By conducting activity assays of the osteocalcin promoter and transcription factor, we found that the OSE2 site in the osteocalcin promoter and Runx2 were important for increased osteocalcin promoter activity by PUGNAc. Furthermore, PUGNAc led to increased O-GlcNAc modification of Runx2, which regulated the transcription of its target gene osteocalcin. Thus, these data provide evidence that O-GlcNAc modification may be a new mode of osteoblastic differentiation regulation.","is_dataset_classified":null,"base_score":3.8712010109078907,"endowment":3.8712010109078907,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17707335","pmcid":null,"openalex_id":"https://openalex.org/W1980360189","authors":[],"funders":[],"total_grants":0,"fwci":0.5032,"citation_percentile":0.62264083,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":3},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2018,"count":1},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":3},{"year":2022,"count":4},{"year":2023,"count":2},{"year":2024,"count":8},{"year":2025,"count":5},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X07016440?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X07016440?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bbrc.2007.07.149","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17707335","host_type":"repository"},{"url":"https://oasis.postech.ac.kr/handle/2014.oak/17451","host_type":"repository"},{"url":"http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34548331814","host_type":"repository"}],"fields_of_study":["Glycosylation and Glycoproteins Research","Galectins and Cancer Biology","RNA modifications and cancer","3T3 Cells","Acetylglucosamine","Animals","Ascorbic Acid","Binding Sites","Blotting, Western","COS Cells","Cell Differentiation","Chlorocebus aethiops","Core Binding Factor Alpha 1 Subunit","Gene Expression Regulation","Luciferases","Mice","Osteoblasts","Osteocalcin","Oximes","Phenylcarbamates","Promoter Regions, Genetic","Protein Binding","RNA, Messenger","Recombinant Fusion Proteins","Reverse Transcriptase Polymerase Chain Reaction","Transfection","beta-N-Acetylhexosaminidases"],"mesh_terms":["Acetylglucosamine","Animals","Ascorbic Acid","beta-N-Acetylhexosaminidases","Binding Sites","Cell Differentiation","Chlorocebus aethiops","Gene Expression Regulation","Luciferases","Osteoblasts","Oximes","Promoter Regions, Genetic","Protein Binding","Recombinant Fusion Proteins","RNA, Messenger","Transfection","Blotting, Western","Osteocalcin","3T3 Cells","COS Cells","Reverse Transcriptase Polymerase Chain Reaction","Phenylcarbamates","Core Binding Factor Alpha 1 Subunit","Mice"],"keywords":["Osteocalcin","RUNX2","Chemistry","Osteoblast","Transcription factor","Parathyroid hormone","Internal medicine","Forskolin","Endocrinology","Cell biology","Gene","Biochemistry","Biology","Receptor","Enzyme","Calcium","Alkaline phosphatase","Medicine","In vitro"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T17:14:01.800997Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}