{"doi":"10.1016/j.bbrc.2007.07.069","title":"Artemisolide is a typical inhibitor of IκB kinase β targeting cysteine-179 residue and down-regulates NF-κB-dependent TNF-α expression in LPS-activated macrophages","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2007,"id":591946,"datarank":1.565238228350393,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":1.0765237476471705,"self_endowment_contribution":0.4887144807032224,"citer_contribution":1.0765237476471705,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":21,"citers_with_citation_signal":21,"citers_with_endowment":21,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1514620,"name":"Jun-Young Lee","orcid":null,"position":1,"is_corresponding":false},{"id":1514621,"name":"Jee Hee Seo","orcid":null,"position":2,"is_corresponding":false},{"id":899988,"name":"Hwa Young Lee","orcid":"0000-0003-1445-9157","position":3,"is_corresponding":false},{"id":1514622,"name":"Shi Yong Ryu","orcid":null,"position":4,"is_corresponding":false},{"id":1514623,"name":"Byung Woo Ahn","orcid":null,"position":5,"is_corresponding":false},{"id":1514625,"name":"Chong-Kil Lee","orcid":null,"position":6,"is_corresponding":false},{"id":1514628,"name":"Bang Yeon Hwang","orcid":null,"position":7,"is_corresponding":false},{"id":1514631,"name":"Sang-Bae Han","orcid":null,"position":8,"is_corresponding":false},{"id":413763,"name":"Youngsoo Kim","orcid":"0000-0002-7592-1387","position":9,"is_corresponding":false},{"id":1514619,"name":"Byung Hak Kim","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Artemisolide is a typical inhibitor of IκB kinase β targeting cysteine-179 residue and down-regulates NF-κB-dependent TNF-α expression in LPS-activated macrophages","abstract":"Nuclear factor (NF)-kappaB regulates a central common signaling for immunity and cell survival. Artemisolide (ATM) was previously isolated as a NF-kappaB inhibitor from a plant of Artemisia asiatica. However, molecular basis of ATM on NF-kappaB activation remains to be defined. Here, we demonstrate that ATM is a typical inhibitor of IkappaB kinase beta (IKKbeta), resulting in inhibition of lipopolysaccharide (LPS)-induced NF-kappaB activation in RAW 264.7 macrophages. ATM inhibited the kinase activity of highly purified IKKbeta and also LPS-induced IKK activity in the cells. Moreover, the effect of ATM on IKKbeta activity was completely abolished by substitution of Cys-179 residue of IKKbeta to Ala residue, indicating direct targeting site of ATM. ATM could inhibit IkappaBalpha phosphorylation in LPS-activated RAW 264.7 cells and subsequently prevent NF-kappaB activation. Further, we demonstrate that ATM down-regulates NF-kappaB-dependent TNF-alpha expression. Taken together, this study provides a pharmacological potential of ATM in NF-kappaB-dependent inflammatory disorders.","is_dataset_classified":null,"base_score":3.258096538021482,"endowment":3.258096538021482,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17669364","pmcid":null,"openalex_id":"https://openalex.org/W2025583948","authors":[],"funders":[],"total_grants":0,"fwci":0.6583,"citation_percentile":0.74436944,"influential_citations":0,"citation_trend":[{"year":2012,"count":5},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X07015069?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X07015069?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bbrc.2007.07.069","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17669364","host_type":"repository"}],"fields_of_study":["NF-κB Signaling Pathways","Natural product bioactivities and synthesis","Immune Response and Inflammation"],"mesh_terms":["Anti-Inflammatory Agents","Cysteine","Dose-Response Relationship, Drug","Enzyme Inhibitors","Lipopolysaccharides","Macrophages","Phosphorylation","Triterpenes","Tumor Necrosis Factor-alpha","Signal Transduction","Down-Regulation","NF-kappa B","I-kappa B Kinase"],"keywords":["NF-κB","IκB kinase","Kinase","Tumor necrosis factor alpha","Phosphorylation","Cysteine","NFKB1","IκBα","P50","Cell biology","Chemistry","Signal transduction","Lipopolysaccharide","Residue (chemistry)","Biochemistry","Biology","Cancer research","Enzyme","Transcription factor","Immunology","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T11:30:55.209273Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}