{"doi":"10.1016/j.bbrc.2004.01.058","title":"A heat shock protein70 fusion protein with α1-antitrypsin in plasma of Type 1 diabetic subjects","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2004,"id":673813,"datarank":0.5495342469194471,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"self_citation_contribution":0.5495342469194471,"citation_network_contribution":0.0,"self_endowment_contribution":0.5495342469194471,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1760512,"name":"Andrea Pagetta","orcid":null,"position":1,"is_corresponding":false},{"id":1760511,"name":"Paola Finotti","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A heat shock protein70 fusion protein with α1-antitrypsin in plasma of Type 1 diabetic subjects","abstract":"The recent observation that heat shock proteins (HSPs), mostly glucose regulated protein94 (Grp94) and HSP70, are present in plasma of Type 1 diabetic subjects as complexes with immunoglobulins, prompted us to investigate the nature and extent of this association, whether it represents HSP-induced activation of the immune system. Two complementary affinity chromatography procedures followed by immunoprecipitation and immunoblot analyses of HSP-enriched, plasma-purified peaks, revealed that HSPs were inextricably linked with IgG in SDS-resistant complexes from which proteins dissociate partially under reducing treatment. HSP70 was found also closely linked with alpha1-antitrypsin (alpha1AT) in a single protein having the mass of alpha1AT but elution characteristics different from those of normal alpha1AT. Immunoprecipitation with anti-HSP70 antibodies led to co-immunoprecipitation of the alpha1AT species linked to HSP70, thus confirming fusion of the proteins. The additional finding of circulating antibodies against the HSP70-alpha1AT protein supported its immunogenic properties with implications for diabetes and its complications.","is_dataset_classified":null,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"14766207","pmcid":null,"openalex_id":"https://openalex.org/W2025296865","authors":[],"funders":[],"total_grants":0,"fwci":0.479,"citation_percentile":0.61173879,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":3},{"year":2014,"count":5},{"year":2016,"count":2},{"year":2017,"count":2},{"year":2018,"count":2},{"year":2020,"count":2},{"year":2021,"count":4},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X04001020?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X04001020?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bbrc.2004.01.058","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/14766207","host_type":"repository"}],"fields_of_study":["Heat shock proteins research","Endoplasmic Reticulum Stress and Disease","thermodynamics and calorimetric analyses","Adult","Blotting, Western","Chromatography, Affinity","Diabetes Mellitus, Type 1","Dose-Response Relationship, Drug","Electrophoresis","Electrophoresis, Polyacrylamide Gel","Female","HSP70 Heat-Shock Proteins","Humans","Immunoblotting","Insulin","Male","Membrane Proteins","Middle Aged","Plasma","Precipitin Tests","Protein Binding","Recombinant Fusion Proteins","Sepharose","alpha 1-Antitrypsin"],"mesh_terms":["Adult","alpha 1-Antitrypsin","Chromatography, Affinity","Diabetes Mellitus, Type 1","Dose-Response Relationship, Drug","Electrophoresis","Electrophoresis, Polyacrylamide Gel","Female","Humans","Insulin","Male","Membrane Proteins","Middle Aged","Plasma","Precipitin Tests","Protein Binding","Recombinant Fusion Proteins","Sepharose","Immunoblotting","Blotting, Western","HSP70 Heat-Shock Proteins"],"keywords":["Heat shock protein","Shock (circulatory)","Fusion","Fusion protein","Chemistry","Internal medicine","Medicine","Biochemistry","Recombinant DNA","Philosophy"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T14:34:11.820289Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}