{"doi":"10.1016/j.bbrc.2003.08.005","title":"Bisulfite conversion-specific and methylation-specific PCR: a sensitive technique for accurate evaluation of CpG methylation","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2003,"id":593962,"datarank":0.637274286307404,"base_score":4.248495242049359,"endowment":4.248495242049359,"self_citation_contribution":0.637274286307404,"citation_network_contribution":0.0,"self_endowment_contribution":0.637274286307404,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":69,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1520266,"name":"Jason Anast","orcid":null,"position":1,"is_corresponding":false},{"id":1520267,"name":"William Bassett","orcid":null,"position":2,"is_corresponding":false},{"id":1520268,"name":"Toshifumi Kawakami","orcid":null,"position":3,"is_corresponding":false},{"id":1520269,"name":"Noriaki Sakuragi","orcid":null,"position":4,"is_corresponding":false},{"id":354820,"name":"Rajvir Dahiya","orcid":null,"position":5,"is_corresponding":false},{"id":1520265,"name":"Masahiro Sasaki","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Bisulfite conversion-specific and methylation-specific PCR: a sensitive technique for accurate evaluation of CpG methylation","abstract":"Methylation-specific PCR (MSP) is frequently used to distinguish methylated alleles in the genome. Sequences that have been incompletely converted during bisulfite treatment are frequently co-amplified during MSP. For accurate MSP, it is important to detect methylated sequences in a background of unconverted DNA with a high level of sensitivity. We report here sensitive techniques, bisulfite conversion-specific MSP (BS-MSP) to accurately evaluate CpG methylation. BS-MSP provides accurate results across a wide spectrum of bisulfite conversion levels. BS-MSP is also confirmed to be a useful technique for the routine analysis of clinical tumor specimens that were paraffin-embedded and microdissected. BS-MSP thus provides the powerful features of ease of use and compatibility with paraffin sections. We recommend that methylation analysis should include a step to eliminate unconverted DNA to avoid overestimation of the DNA methylation level in the samples.","is_dataset_classified":null,"base_score":4.248495242049359,"endowment":4.248495242049359,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12951050","pmcid":null,"openalex_id":"https://openalex.org/W2060132371","authors":[],"funders":[{"funder_name":"NIA NIH HHS","grant_id":"R01 AG 016870","title":null},{"funder_name":"NIA NIH HHS","grant_id":"R01 AG 21418","title":null}],"total_grants":2,"fwci":1.0838,"citation_percentile":0.74904626,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":4},{"year":2014,"count":6},{"year":2015,"count":8},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":2},{"year":2020,"count":2},{"year":2021,"count":4},{"year":2022,"count":4},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":1}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X03015894?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X03015894?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bbrc.2003.08.005","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12951050","host_type":"repository"}],"fields_of_study":["Epigenetics and DNA Methylation","Cancer-related gene regulation","RNA modifications and cancer"],"mesh_terms":["Urinary Bladder Neoplasms","DNA, Neoplasm","Reference Values","Sensitivity and Specificity","Sulfites","Restriction Mapping","Reproducibility of Results","Cadherins","Polymerase Chain Reaction","Sequence Analysis, DNA","CpG Islands","DNA Methylation"],"keywords":["Bisulfite","Bisulfite sequencing","Methylation","Illumina Methylation Assay","DNA methylation","CpG site","Molecular biology","Methylated DNA immunoprecipitation","Biology","DNA","Computational biology","Genetics","Gene","Gene expression"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T12:59:52.949595Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}