{"doi":"10.1016/j.bbmt.2006.12.336","title":"331: Global assessment of B cell alloimmunity using microarrays of 5000 human proteins","abstract":"Allogeneic Hematopoietic Cell Transplantation (HCT) can cure hematologic malignancies through beneficial graft-v-leukemia (GVL) allo-immune responses, but is limited by graft-versus-host disease (GVHD). Recent studies demonstrate allogeneic antibodies (Ab) develop against minor histocompatibility antigens (mHA) encoded on the Y-chromosome (H-Y antigens) develop after sex-mismatch HCT in association with chronic GVHD and persistent disease remission. We hypothesize that novel mHA can be serologically identified as targets of allo-Ab responses that develop post-transplant but are absent pre-transplant. ProtoArray™ (Invitrogen) displays 5,000 full-length human proteins with N-terminal GST epitopes expressed in baculovirus and affinity purified under native conditions maintaining their cellular enzymatic activities/native conformations. Technical replicates of 8 plasma measured at 1:50, 1:150, 1:500 provided correlation coeffients, R2=0.94-0.97 confirming technical feasibility. In order to identify targets of allo-Ab, pretransplant fluorescent signal intensities were subtracted from their one-year plasma results for all 5000 antigens. While Ab responses were unchanged for 4600 (92%) antigens, new allo-Ab responses targeted 60-75 antigens with fluorescent differences ranging 0.5 to 3 logs. In comparison with their respective donor results, 30-40% appear to result from adoptive donor transfer while the remaining develop de novo . Over 90% of allo-Ab targets have known non-synonymous SNP which when disparate in donor and recipient may elicit alloimmunity and this genotyping is ongoing. No single protein was recognized by \"new\" Ab responses in all patients, however polymorphic proteins Growth Arrest Specific-7 (GAS7), laminin A/C, and ribosomal protein S19 (RPS19) were recognized by two of the four patients after HCT. Results from plasma samples collected 3, 6, 9 and 12 months after HCT demonstrate the progression of alloimmune immune responses. Though these novel mHA require validation by large clinically characterized patient samples, protein microarrays are innovative, powerful tools for high-throughput global assessment of B -cell alloimmunity after HCT providing sufficient reproducibility for candidate mHA discovery.","journal":"Biology of Blood and Marrow Transplantation","year":2007,"id":3503,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0405,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2007-02-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":36528,"name":"L. Weintraub","orcid":null,"position":2,"is_corresponding":false},{"id":36529,"name":"D. Miklos","orcid":null,"position":4,"is_corresponding":false},{"id":19327,"name":"Persis P. Wadia","orcid":null,"position":5,"is_corresponding":false},{"id":19328,"name":"Marc Coram","orcid":"0000-0002-8877-2501","position":6,"is_corresponding":false},{"id":36530,"name":"Lauren Weintraub","orcid":"0009-0009-4406-1255","position":7,"is_corresponding":false},{"id":51,"name":"Atul Janardhan Butte","orcid":"0000-0002-7433-2740","position":8,"is_corresponding":false},{"id":36531,"name":"David Miklos","orcid":null,"position":9,"is_corresponding":false}],"reference_count":0,"raw_metadata":null,"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}