{"doi":"10.1016/j.bbamcr.2025.120057","title":"Knockdown of translocon-associated protein subunit beta (TRAPβ) stimulates cell cycle arrest and apoptosis in human colorectal cancer cells","abstract":null,"journal":"Biochimica et Biophysica Acta (BBA) - Molecular Cell Research","year":2025,"id":674524,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":588148,"name":"Jing Peng","orcid":"0000-0001-5490-6228","position":1,"is_corresponding":false},{"id":1762369,"name":"Cheng-I Wei","orcid":null,"position":2,"is_corresponding":false},{"id":1762370,"name":"Seong-Ho Lee","orcid":null,"position":3,"is_corresponding":false},{"id":1762368,"name":"Darshika Amarakoon","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Knockdown of translocon-associated protein subunit beta (TRAPβ) stimulates cell cycle arrest and apoptosis in human colorectal cancer cells","abstract":"Translocon-associated protein subunit beta (TRAPβ), also known as signal sequence receptor 2 (SSR2) serves as an auxiliary protein facilitating co-translational translocation in the endoplasmic reticulum (ER); however, its role in colorectal cancer is unknown to date. The objectives of the current study are to examine if TRAPβ/SSR2 knockdown affects the cell proliferation and to elucidate mechanisms by which TRAPβ/SSR2 regulates proliferation of human colorectal cancer. We silenced TRAPβ/SSR2 transiently and stably in human colorectal cancer cell lines and analyzed cell proliferative properties. Transient transfection of TRAPβ/SSR2 siRNA significantly repressed the viability of five different types of human colorectal cancer cells. Flow cytometry and western blot showed that TRAPβ/SSR2 knockdown led to significant increase of G2/M-phase arrest in SW480 cells and S-phase arrest in HCT116 and DLD-1 cells. Annexin V-fluorescein isothiocyanate and propidium iodide staining showed that TRAPβ/SSR2 knockdown significantly induced apoptosis in SW480, HCT116, and DLD-1 cells. Similarly, SW480 stable cells with TRAPβ/SSR2 knockdown showed a significant inhibition of anchorage-independent cell growth, an increase of G2/M-phase arrest with downregulation of cyclin B1, and increase of apoptosis. Regarding mechanisms, TRAPβ/SSR2 knockdown mitigated epidermal growth factor-stimulated activation of mitogen-activated protein kinase (MAPK) pathways and showed significantly decreased expression of inositol-requiring enzyme 1 alpha (IRE1α), while IRE1α reintroduction in TRAPβ/SSR2 knockdown cells reversed G2/M-phase arrest and promoted cell cycle progression. All taken together, our data demonstrate that TRAPβ/SSR2 in the ER could be a molecular target to control cell cycle progression and apoptosis through MAPK-mediated and IRE1α-mediated pathways in human colorectal cancer cells.","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"40925508","pmcid":null,"openalex_id":"https://openalex.org/W4414042351","authors":[],"funders":[{"funder_name":"National Institute of Food and Agriculture","grant_id":"","title":null},{"funder_name":"U.S. Department of Agriculture","grant_id":"","title":null}],"total_grants":2,"fwci":1.3869,"citation_percentile":0.80835016,"influential_citations":0,"citation_trend":[{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"http://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0167488925001624?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0167488925001624?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.bbamcr.2025.120057","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/40925508","host_type":"repository"}],"fields_of_study":["Endoplasmic Reticulum Stress and Disease","Heat shock proteins research","Cell death mechanisms and regulation","Medicine","Biology","Humans","Colorectal Neoplasms","Apoptosis","Cell Proliferation","Cell Line, Tumor","Cell Cycle Checkpoints","HCT116 Cells","Gene Knockdown Techniques","Gene Expression Regulation, Neoplastic","Endoribonucleases","Protein Serine-Threonine Kinases"],"mesh_terms":["Endoribonucleases","Humans","Colorectal Neoplasms","Gene Expression Regulation, Neoplastic","Apoptosis","Protein Serine-Threonine Kinases","HCT116 Cells","Cell Line, Tumor","Cell Proliferation","Gene Knockdown Techniques","Cell Cycle Checkpoints"],"keywords":["Gene knockdown","Propidium iodide","Apoptosis","Cell growth","Cell cycle","Cell cycle checkpoint","Transfection","Annexin","Endoplasmic reticulum","Colorectal Cancer","Inositol-requiring Enzyme 1 Alpha","Signal Sequence Receptor 2","Translocon-associated Protein Subunit Beta"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T17:11:10.616773Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}