{"doi":"10.1016/j.bbadis.2023.166686","title":"Charged multivesicular body protein 2B ameliorates biliary injury in the liver from donation after cardiac death rats via autophagy with air-oxygenated normothermic machine perfusion","abstract":null,"journal":"Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease","year":2023,"id":628201,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1626362,"name":"Ji-Hua Shi","orcid":null,"position":1,"is_corresponding":false},{"id":430244,"name":"Qi Liu","orcid":"0000-0002-5684-7396","position":2,"is_corresponding":false},{"id":1626363,"name":"Dong-Jing Yang","orcid":null,"position":3,"is_corresponding":false},{"id":1626364,"name":"Zhi-Ping Yan","orcid":null,"position":4,"is_corresponding":false},{"id":1626365,"name":"Jia-Kai Zhang","orcid":null,"position":5,"is_corresponding":false},{"id":1626366,"name":"Hong-Wei Tang","orcid":null,"position":6,"is_corresponding":false},{"id":1626367,"name":"Wen-Zhi Guo","orcid":null,"position":7,"is_corresponding":false},{"id":103926,"name":"Yang Jin","orcid":"0000-0003-2409-7073","position":8,"is_corresponding":false},{"id":1626368,"name":"Shui-Jun Zhang","orcid":null,"position":9,"is_corresponding":false},{"id":34508,"name":"Yang Bai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Charged multivesicular body protein 2B ameliorates biliary injury in the liver from donation after cardiac death rats via autophagy with air-oxygenated normothermic machine perfusion","abstract":"Normothermic machine perfusion (NMP) could provide a curative treatment to reduce biliary injury in donation after cardiac death (DCD) donor livers; however, the underlying mechanisms remain poorly understood. In a rat model, our study compared air-oxygenated NMP to hyperoxygenated NMP and found that air-oxygenated NMP improved DCD functional recovery. Here, we found that the charged multivesicular body protein 2B (CHMP2B) expression was substantially elevated in the intrahepatic biliary duct endothelium of the cold-preserved rat DCD liver after air-oxygenated NMP or in biliary endothelial cells under hypoxia/physoxia. CHMP2B knockout (CHMP2B−/−) rat livers showed increased biliary injury after air-oxygenated NMP, indicated by decreased bile production and bilirubin level, elevated biliary levels of lactate dehydrogenase and gamma-glutamyl transferase. Mechanically, we demonstrated that CHMP2B was transcriptionally regulated by Kruppel-like transcription factor 6 (KLF6) and alleviated biliary injury through decreasing autophagy. Collectively, our results suggested that air-oxygenated NMP regulates CHMP2B expression through the KLF6, which reduces biliary injury by inhibiting autophagy. Targeting the KLF6-CHMP2B autophagy axis may provide a solution to reducing biliary injury in DCD livers undergoing NMP.","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36907288","pmcid":null,"openalex_id":"https://openalex.org/W4323928605","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"81971881","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"82170670","title":null},{"funder_name":"Henan Provincial Science and Technology Research Project","grant_id":"SB201901045","title":null}],"total_grants":3,"fwci":2.2284,"citation_percentile":0.87165823,"influential_citations":0,"citation_trend":[{"year":2024,"count":1},{"year":2025,"count":3},{"year":2026,"count":4}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://doi.org/10.1016/j.bbadis.2023.166686","host_type":"journal"},{"url":"https://doi.org/10.1016/j.bbadis.2023.166686","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0925443923000522?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0925443923000522?httpAccept=text/plain","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36907288","host_type":"repository"}],"fields_of_study":["Organ Transplantation Techniques and Outcomes","Liver Disease and Transplantation","Liver Disease Diagnosis and Treatment","Rats","Animals","Endothelial Cells","Multivesicular Bodies","Liver Transplantation","Organ Preservation","Liver","Perfusion","Death"],"mesh_terms":["Animals","Death","Liver","Organ Preservation","Perfusion","Liver Transplantation","Endothelial Cells","Rats","Multivesicular Bodies"],"keywords":["Autophagy","Liver injury","Hypoxia (environmental)","Medicine","Reperfusion injury","Liver transplantation","Chemistry","Internal medicine","Pharmacology","Biochemistry","Ischemia","Transplantation","Oxygen","Apoptosis","Donation After Cardiac Death","Kruppel-like Transcription Factor 6","Normothermic Machine Perfusion","Charged Multivesicular Body Protein 2B"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T11:47:47.283574Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}