{"doi":"10.1016/j.ajpath.2016.01.011","title":"A Unique Role for Endothelial Cell Kinesin Light Chain 1, Variant 1 in Leukocyte Transendothelial Migration","abstract":null,"journal":"The American Journal of Pathology","year":2016,"id":659164,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1720697,"name":"William A. Muller","orcid":null,"position":1,"is_corresponding":false},{"id":1720696,"name":"Bita F. Cyrus","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Unique Role for Endothelial Cell Kinesin Light Chain 1, Variant 1 in Leukocyte Transendothelial Migration","abstract":"A reservoir of parajunctional membrane in endothelial cells, the lateral border recycling compartment (LBRC), is critical for transendothelial migration (TEM). We have previously shown that targeted recycling of the LBRC to the site of TEM requires microtubules and a kinesin molecular motor. However, the identity of the kinesin and mechanism of cargo binding were not known. We show that microinjection of endothelial cells with a monoclonal antibody specific for kinesin-1 significantly blocked LBRC-targeted recycling and TEM. In complementary experiments, knocking down KIF5B, a ubiquitous kinesin-1 isoform, in endothelial cells significantly decreased targeted recycling of the LBRC and leukocyte TEM. Kinesin heavy chains move cargo along microtubules by one of many kinesin light chains (KLCs), which directly bind the cargo. Knocking down KLC 1 isoform variant 1 (KLC1C) significantly decreased LBRC-targeted recycling and TEM, whereas knocking down other isoforms of KLC1 had no effect. Re-expression of KLC1C resistant to the knockdown shRNA restored targeted recycling and TEM. Thus kinesin-1 and KLC1C are specifically required for targeted recycling and TEM. These data suggest that of the many potential combinations of the 45 kinesin family members and multiple associated light chains, KLC1C links the LBRC to kinesin-1 (KIF5B) during targeted recycling and TEM. Thus, KLC1C can potentially be used as a target for anti-inflammatory therapy.","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26994343","pmcid":"PMC4861765","openalex_id":"https://openalex.org/W2301892834","authors":[],"funders":[{"funder_name":"NIH","grant_id":"F31HL114374","title":null},{"funder_name":"NIH","grant_id":"R01 HL046849","title":null},{"funder_name":"NIH","grant_id":"R37 HL064774","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL064774","title":null}],"total_grants":4,"fwci":0.5643,"citation_percentile":0.65658302,"influential_citations":0,"citation_trend":[{"year":2016,"count":2},{"year":2019,"count":2},{"year":2020,"count":2},{"year":2023,"count":2},{"year":2024,"count":1},{"year":2025,"count":4}],"oa_status":"bronze","license":"http://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"http://ajp.amjpathol.org/article/S0002944016001024/pdf","host_type":"journal"},{"url":"http://ajp.amjpathol.org/article/S0002944016001024/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0002944016001024?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0002944016001024?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ajpath.2016.01.011","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26994343","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4861765","host_type":"repository"}],"fields_of_study":["Phagocytosis and Immune Regulation","Cellular Mechanics and Interactions","Microtubule and mitosis dynamics","Antibodies, Monoclonal","Gene Knockdown Techniques","Human Umbilical Vein Endothelial Cells","Humans","Inflammation","Kinesins","Leukocytes","Microinjections","Microtubule-Associated Proteins","Microtubules","Point Mutation","Transduction, Genetic","Transendothelial and Transepithelial Migration"],"mesh_terms":["Antibodies, Monoclonal","Humans","Inflammation","Leukocytes","Microinjections","Microtubule-Associated Proteins","Microtubules","Transduction, Genetic","Kinesins","Point Mutation","Gene Knockdown Techniques","Transendothelial and Transepithelial Migration","Human Umbilical Vein Endothelial Cells"],"keywords":["Kinesin","Cell biology","Immunoglobulin light chain","Endothelial stem cell","Cell","Chemistry","Microtubule","Biology","Immunology","Biochemistry","In vitro"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T06:18:22.524426Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}