{"doi":"10.1016/j.aca.2024.342532","title":"MazF-rolling circle amplification combined MALDI-TOF MS for site-specific detection of N6-methyladenosine RNA","abstract":null,"journal":"Analytica Chimica Acta","year":2024,"id":617639,"datarank":0.47620510128367954,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.13081733733457265,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.13081733733457265,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":9,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1592941,"name":"Qiuyuan Lin","orcid":null,"position":1,"is_corresponding":false},{"id":167546,"name":"Jia Yi","orcid":null,"position":2,"is_corresponding":false},{"id":1377352,"name":"Qian Lyu","orcid":"0009-0002-0198-6433","position":3,"is_corresponding":false},{"id":1592944,"name":"Qingwei Ma","orcid":null,"position":4,"is_corresponding":false},{"id":1200233,"name":"Liang Qiao","orcid":"0000-0002-6233-8459","position":5,"is_corresponding":false},{"id":1592940,"name":"Guobin Han","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"MazF-rolling circle amplification combined MALDI-TOF MS for site-specific detection of N6-methyladenosine RNA","abstract":"N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is one of the most abundant chemical modifications in RNA and has vital significance in cellular processes and tumor development. However, the accurate analysis of site-specific m<sup>6</sup>A modification remains a challenge. In this work, a MazF endoribonuclease activated rolling circle amplification (MazF-RCA) combined MALDI-TOF MS assay is developed for the detection of site-specific m<sup>6</sup>A-RNA. MazF endoribonuclease can specifically cleave the ACA motif, leaving methylated (m<sup>6</sup>A)CA motif intact. The intact methylated RNA can then be amplified through rolling circle amplification, and the generated reporter oligonucleotides are detected by MALDI-TOF MS. The assay exhibits good quantification ability, presenting a wide linear range (100 fM to 10 nM) with the limit-of-detection lower than 100 fM. Additionally, the assay can accurately detect methylated RNA in the presence of large amount of non-methylated RNA with a relative abundance of methylated RNA down to 0.5%. The developed assay was further applied to detect m<sup>6</sup>A-RNA spiked in MCF-7 cell RNA extracts, with the recovery rates in the range of 90.64-106.93%. The present assay provides a novel platform for the analysis of site-specific m<sup>6</sup>A-RNA at high specificity and sensitivity, which can promote the study of RNA methylation in clinical and biomedical research.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38609270","pmcid":null,"openalex_id":"https://openalex.org/W4393151738","authors":[],"funders":[{"funder_name":"National Key Research and Development Program of China","grant_id":"2022YFC2704300","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"22074022","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"22374031","title":null},{"funder_name":"Ministry of Science and Technology of the People&apos;s Republic of China","grant_id":"","title":null}],"total_grants":4,"fwci":1.4712,"citation_percentile":0.80867451,"influential_citations":0,"citation_trend":[{"year":2024,"count":2},{"year":2025,"count":5},{"year":2026,"count":2}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0003267024003337?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0003267024003337?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.aca.2024.342532","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38609270","host_type":"repository"}],"fields_of_study":["RNA modifications and cancer"],"mesh_terms":["Adenosine","Endoribonucleases","RNA","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization"],"keywords":["RNA","Endoribonuclease","N6-Methyladenosine","Chemistry","RNA methylation","Molecular biology","Oligonucleotide","Methylation","Nuclease protection assay","Biochemistry","Non-coding RNA","DNA","Gene","Biology","Methyltransferase","MALDI-TOF MS","rolling circle amplification","N(6)-methyladenosine","Mazf Endoribonuclease"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T02:15:51.717915Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}