{"doi":"10.1016/j.aca.2018.02.046","title":"A quantitative metabolomics study of bacterial metabolites in different domains","abstract":null,"journal":"Analytica Chimica Acta","year":2018,"id":603243,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":335217,"name":"Mengyang Xu","orcid":"0000-0002-4487-7088","position":1,"is_corresponding":false},{"id":1547467,"name":"Patrick Metz","orcid":null,"position":2,"is_corresponding":false},{"id":1547469,"name":"Pratiti Ghosh-Dastidar","orcid":null,"position":3,"is_corresponding":false},{"id":293945,"name":"Jiangjiang Zhu","orcid":"0000-0002-4548-8949","position":4,"is_corresponding":false},{"id":296430,"name":"Fanyi Zhong","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A quantitative metabolomics study of bacterial metabolites in different domains","abstract":"Absolute metabolite concentrations are essential information for quantitative metabolomics studies, as concentrations are closely related to metabolic reactions, enzyme kinetics and other important biological activities. A well-performed metabolites extraction procedure, a reliable detection method, and a robust quantitative approach are all critical factors for obtaining absolute metabolite concentrations. Here, we used a HPLC-MS/MS based platform to successfully develop a <sup>13</sup>C-labeled quantitative metabolomics approach, and applied this novel method to quantify bacterial metabolite concentrations in three different domains (i.e., intracellularly, extracellularly and to the whole culture), with high accuracy for a model Escherichia coli bacteria. The bacterial culture was grown in universal <sup>13</sup>C-labeled medium and the metabolites were extracted by 40/40/20/methanol/ACN/H<sub>2</sub>O with 0.1% formic acid. One hundred and twenty-five metabolites were initially screened and one hundred and six <sup>13</sup>C metabolites were confidently detected from the model bacteria grown in <sup>13</sup>C-labeled medium. A subset of twenty-one metabolites was subsequently quantified using <sup>12</sup>C-metabolite chemical standards to assist the calculation of <sup>13</sup>C metabolite concentration. This rigorous <sup>13</sup>C-labled quantitative method was then applied to characterize the metabolic profile changes in three domains of E. coli going through antibiotic treatment. Our results demonstrated that metabolites from all three domains can be used to significantly differentiate the ampicillin treatment group and control group (without ampicillin). In conclusion, our work demonstrated that the quantitative metabolomics approach can be used as a valuable tool to study bacterial metabolism in different domains and to understand their response to environmental perturbations.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30292298","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"University of Miami","grant_id":"","title":null}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":null,"license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0003267018302617?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0003267018302617?httpAccept=text/plain","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Escherichia coli","Chromatography, High Pressure Liquid","Tandem Mass Spectrometry","Metabolomics"],"keywords":["Bacterial Metabolism","Quantitative Metabolomics","Intracellular Metabolites","Extracellular Metabolites","Antibiotics Induced Metabolic Shift"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T21:25:38.612664Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}