{"doi":"10.1016/j.abb.2014.01.006","title":"CK2 involvement in ESCRT-III complex phosphorylation","abstract":null,"journal":"Archives of Biochemistry and Biophysics","year":2014,"id":637083,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201610,"name":"Camilla Raiborg","orcid":null,"position":1,"is_corresponding":false},{"id":330008,"name":"Phyllis I. Hanson","orcid":"0000-0001-7983-6668","position":2,"is_corresponding":false},{"id":201606,"name":"Coen Campsteijn","orcid":null,"position":3,"is_corresponding":false},{"id":236757,"name":"Harald Stenmark","orcid":"0000-0002-1971-4252","position":4,"is_corresponding":false},{"id":1653979,"name":"Lorenzo A. Pinna","orcid":null,"position":5,"is_corresponding":false},{"id":1653978,"name":"Mauro Salvi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CK2 involvement in ESCRT-III complex phosphorylation","abstract":"The multivesicular body (MVB) sorting pathway is a mechanism for delivering transmembrane proteins into the lumen of the lysosome for degradation. ESCRT-III is the final complex in the pathway that assembles on endosomes and executes membrane scission of intraluminal vesicles. In addition, proteins of this complex are involved in other topologically similar processes such as cytokinesis, virus egress and autophagy. Here we show that protein kinase CK2α is involved in the phosphorylation of the ESCRT-III subunits CHMP3 and CHMP2B, as well as of VPS4B/SKD1, an ATPase that mediates ESCRT-III disassembly. This phosphorylation is observed both in vitro and in cells. While we do not observe recruitment of CK2α to endosomes, we demonstrate the localization of CK2α to midbodies during cytokinesis. Phosphomimetic and non-phosphorylatable mutants of ESCRT-III proteins can still bind endosomes and localize to midbodies, indicating that CK2α does not regulate ESCRT-III localization. Finally, we analyzed two cellular functions where CHMP3, CHMP2B and VPS4 are known to be involved, epidermal growth factor degradation and cytokinetic abscission. We demonstrate that the former is impaired by CK2α downregulation whereas the latter is not affected. Taken together, our results indicate that CK2α regulates the function of ESCRT-III proteins in MVB sorting.","is_dataset_classified":null,"base_score":2.772588722239781,"endowment":2.772588722239781,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24440309","pmcid":null,"openalex_id":"https://openalex.org/W2009164057","authors":[],"funders":[{"funder_name":"European Commission","grant_id":"233146","title":"The PI3K-III complex: Function in cell regulation and tumour suppression"}],"total_grants":1,"fwci":0.8441,"citation_percentile":0.68522582,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2016,"count":5},{"year":2018,"count":2},{"year":2019,"count":3},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2024,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0003986114000162?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0003986114000162?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.abb.2014.01.006","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24440309","host_type":"repository"},{"url":"http://hdl.handle.net/11577/2833317","host_type":"repository"},{"url":"http://dx.doi.org/10.1016/j.abb.2014.01.006","host_type":""},{"url":"https://dx.doi.org/10.1016/j.abb.2014.01.006","host_type":""},{"url":"https://hdl.handle.net/11577/2833317","host_type":""}],"fields_of_study":["Cellular transport and secretion","Phagocytosis and Immune Regulation","Autophagy in Disease and Therapy","0301 basic medicine","0303 health sciences","03 medical and health sciences","ATPases Associated with Diverse Cellular Activities","Adenosine Triphosphatases","Casein Kinase II","Down-Regulation","Endosomal Sorting Complexes Required for Transport","Endosomes","Epidermal Growth Factor","HEK293 Cells","HeLa Cells","Humans","Phosphorylation"],"mesh_terms":["ATPases Associated with Diverse Cellular Activities","Adenosine Triphosphatases","Epidermal Growth Factor","HeLa Cells","Humans","Phosphorylation","Endosomes","Down-Regulation","Casein Kinase II","Endosomal Sorting Complexes Required for Transport","HEK293 Cells"],"keywords":["ESCRT","Endosome","Cell biology","Cytokinesis","Vacuolar protein sorting","Phosphorylation","TSG101","Biology","Transport protein","Transmembrane protein","Chemistry","Biochemistry","Cell division","Cell","Intracellular","Adenosine Triphosphatases","Endosomal Sorting Complexes Required for Transport","Epidermal Growth Factor","Down-Regulation","Endosomes","HEK293 Cells","ATPases Associated with Diverse Cellular Activities","Humans","Casein Kinase II","HeLa Cells","CHMP2B","CHMP3","Protein kinase CK2","VPS4"],"sdg_mappings":[{"sdg_number":2,"sdg_label":"2. 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