{"doi":"10.1016/j.abb.2004.02.036","title":"Augmentation of lipolysis in adipocytes from fed rats, but not from starved rats, by inhibition of rolipram-sensitive phosphodiesterase 4","abstract":null,"journal":"Archives of Biochemistry and Biophysics","year":2004,"id":659275,"datarank":0.4566783656585135,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.0,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1720979,"name":"Naomichi Okamura","orcid":null,"position":1,"is_corresponding":false},{"id":211148,"name":"Yasushi Kawakami","orcid":null,"position":2,"is_corresponding":false},{"id":193336,"name":"Jiro Nakamura","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Augmentation of lipolysis in adipocytes from fed rats, but not from starved rats, by inhibition of rolipram-sensitive phosphodiesterase 4","abstract":"The sensitivity of adipocytes to lipolytic agents is increased after starvation. In this study, we found that LY294002, an inhibitor of phosphatidylinositol-3 kinase (PI3K), in the concentration of more than 50 microM potentiates lipolysis induced by adenosine deaminase in adipocytes from fed rats (f-adipocytes), but not from starved rats (s-adipocytes). It also enhanced the sensitivity to lipolytic action of isoproterenol in f-adipocytes much more than s-adipocytes. The target of LY294002 may be an anti-lipolytic regulator expressed in response to food intake. Since another PI3K inhibitor, wortmannin, or a phosphodiesterase 3 (PDE3) inhibitor, cilostamide, failed to cause any specific effect to f-adipocytes, the PI3K-PDE3B pathway cannot be a target of LY294002. We found that LY294002 inhibits efficiently the cytoplasmic PDE activity of adipocytes. Rolipram, a specific inhibitor of PDE4, also inhibited the cytoplasmic PDE and caused a preferential increase of lipolysis in f-adipocytes. LY294002 blunted the actions of rolipram on lipolysis and the PDE activity. LY294002 accelerated protein kinase A activation. These data suggest that the rolipram-sensitive PDE4 is an anti-lipolytic enzyme expressed according to food intake. LY294002 may potentiate lipolysis through inhibition of the PDE4.","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15081899","pmcid":null,"openalex_id":"https://openalex.org/W2047412149","authors":[],"funders":[],"total_grants":0,"fwci":0.4541,"citation_percentile":0.59550618,"influential_citations":0,"citation_trend":[{"year":2014,"count":3},{"year":2017,"count":1},{"year":2019,"count":2},{"year":2022,"count":2},{"year":2025,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0003986104001262?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0003986104001262?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.abb.2004.02.036","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15081899","host_type":"repository"}],"fields_of_study":["Phosphodiesterase function and regulation","Diabetes Treatment and Management","Receptor Mechanisms and Signaling","Chemistry","Medicine","Biology","3',5'-Cyclic-AMP Phosphodiesterases","Adipocytes","Animals","Chromones","Cyclic AMP-Dependent Protein Kinases","Cyclic Nucleotide Phosphodiesterases, Type 4","Lipolysis","Morpholines","Phosphoinositide-3 Kinase Inhibitors","Quinolones","Rats","Rolipram","Starvation"],"mesh_terms":["Phosphoinositide-3 Kinase Inhibitors","Animals","Chromones","Lipolysis","Morpholines","Starvation","3',5'-Cyclic-AMP Phosphodiesterases","Quinolones","Adipocytes","Cyclic AMP-Dependent Protein Kinases","Rolipram","Rats","Cyclic Nucleotide Phosphodiesterases, Type 4"],"keywords":["Rolipram","Lipolysis","Phosphodiesterase","Internal medicine","Endocrinology","Chemistry","Phosphodiesterase inhibitor","Enzyme","Biochemistry","Adipose tissue","Biology","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T06:32:08.116630Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}