{"doi":"10.1016/j.ab.2011.12.028","title":"In-microbe formation of nucleotide sugars in engineered Escherichia coli","abstract":null,"journal":"Analytical Biochemistry","year":2012,"id":632625,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":658609,"name":"Yael Bar-Peled","orcid":"0000-0002-4904-5571","position":1,"is_corresponding":false},{"id":1639913,"name":"James Amor Smith","orcid":null,"position":2,"is_corresponding":false},{"id":365962,"name":"John Glushka","orcid":"0000-0002-6615-0496","position":3,"is_corresponding":false},{"id":1639914,"name":"Maor Bar-Peled","orcid":null,"position":4,"is_corresponding":false},{"id":486366,"name":"Ting Yang","orcid":"0000-0001-8370-131X","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"In-microbe formation of nucleotide sugars in engineered Escherichia coli","abstract":"Numerous different nucleotide sugars are used as sugar donors for the biosynthesis of glycans by bacteria, humans, fungi, and plants. However, many of these nucleotide sugars are not available either in their native form or with the sugar portion labeled with a stable or radioactive isotope. Here we demonstrate the use of Escherichia coli metabolically engineered to contain genes that encode proteins that convert monosaccharides into their respective monosaccharide-1-phosphates and subsequently into the corresponding nucleotide sugars. In this system, which we designated \"in-microbe\", reactions occur within 2 to 4 h and can be used to generate nucleotide sugars in amounts ranging from 5 to 12.5 μg/ml cell culture. We show that the E. coli can be engineered to produce the seldom observed nucleotide sugars UDP-2-acetamido-2-deoxy-glucuronic acid (UDP-GlcNAcA) and UDP-2-acetamido-2-deoxy-xylose (UDP-XylNAc). Using similar strategies, we also engineered E. coli to synthesize UDP-galacturonic acid (UDP-GalA) and UDP-galactose (UDP-Gal). ¹³C- and ¹⁵N-labeled NDP-sugars are formed using [¹³C] glucose as the carbon source and with [¹⁵N]NH₄Cl as the nitrogen source.","is_dataset_classified":null,"base_score":2.833213344056216,"endowment":2.833213344056216,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22244806","pmcid":null,"openalex_id":"https://openalex.org/W2134855082","authors":[],"funders":[{"funder_name":"National Science Foundation","grant_id":"IOB-0453664","title":null},{"funder_name":"Office of Biological and Environmental Research in the Department of Energy’s Office of Science","grant_id":"","title":null}],"total_grants":2,"fwci":0.3651,"citation_percentile":0.60234102,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":2},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2022,"count":2},{"year":2025,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0003269711007998?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0003269711007998?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ab.2011.12.028","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22244806","host_type":"repository"}],"fields_of_study":["Glycosylation and Glycoproteins Research","Carbohydrate Chemistry and Synthesis","Biofuel production and bioconversion","Base Sequence","Carbohydrate Metabolism","DNA Primers","Escherichia coli","Genetic Engineering","Nucleotides","Uridine Diphosphate Sugars"],"mesh_terms":["Base Sequence","Escherichia coli","Genetic Engineering","Nucleotides","Uridine Diphosphate Sugars","DNA Primers","Carbohydrate Metabolism"],"keywords":["Nucleotide sugar","Escherichia coli","Nucleotide","Monosaccharide","Biochemistry","Xylose","Chemistry","Glucuronic acid","Galactose","Sugar","Bacteria","Glycan","Sugar acids","Biosynthesis","Biology","Gene","Polysaccharide","Fermentation","Genetics","Glycoprotein"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T10:05:18.811224Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}