{"doi":"10.1016/j.ab.2011.02.011","title":"Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation","abstract":null,"journal":"Analytical Biochemistry","year":2011,"id":591778,"datarank":0.7695978998686313,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.4099136089488755,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.4099136089488755,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":9,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1514205,"name":"Mari Tabuchi","orcid":null,"position":1,"is_corresponding":false},{"id":759393,"name":"Takayuki Inoue","orcid":"0009-0009-9920-644X","position":2,"is_corresponding":false},{"id":1514206,"name":"Toshihiro Yasuda","orcid":null,"position":3,"is_corresponding":false},{"id":652887,"name":"Yasuhisa Fujita","orcid":"0000-0002-1720-3942","position":4,"is_corresponding":false},{"id":1514209,"name":"Haruo Takeshita","orcid":null,"position":5,"is_corresponding":false},{"id":1514204,"name":"Junko Fujihara","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation","abstract":"Deoxyribonuclease I (DNase I) activity in serum has been shown to be a novel diagnostic marker for the early detection of acute myocardial infarction (AMI). However, the conventional method to measure DNase I activity is time-consuming. In the current study, to develop a rapid assay method for DNase I activity for clinical purposes, a microchip electrophoresis device was used to measure DNase I activity. Because DNase I is an endonuclease that degrades double-stranded DNA endo-nucleolytically to produce oligonucleotides, degradation of the DNA standard caused by DNase I action was detected using microchip electrophoresis. We detected DNase I activity within 10 min. This is the first study to apply microchip electrophoresis for the detection of DNase I activity; furthermore, it seems plausible that reduction of analysis time for DNase I activity could make this novel assay method using microchip electrophoresis applicable in clinical use.","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21316332","pmcid":null,"openalex_id":"https://openalex.org/W1996671895","authors":[],"funders":[],"total_grants":0,"fwci":0.6327,"citation_percentile":0.69216525,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0003269711000984?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0003269711000984?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ab.2011.02.011","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21316332","host_type":"repository"}],"fields_of_study":["Microfluidic and Capillary Electrophoresis Applications","Advanced biosensing and bioanalysis techniques","Microfluidic and Bio-sensing Technologies"],"mesh_terms":["Acute Disease","Deoxyribonuclease I","DNA","Humans","Myocardial Infarction","Electrophoresis, Microchip"],"keywords":["Deoxyribonuclease I","Deoxyribonuclease","Endonuclease","DNA","Electrophoresis","Molecular biology","Gel electrophoresis","Hypersensitive site","DNase I hypersensitive site","Oligonucleotide","Chemistry","Chromatography","Biology","Biochemistry","Base sequence"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T10:41:39.151214Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}