{"doi":"10.1016/j.ab.2010.08.028","title":"Nanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies","abstract":null,"journal":"Analytical Biochemistry","year":2011,"id":679232,"datarank":0.62147020895873,"base_score":4.143134726391533,"endowment":4.143134726391533,"self_citation_contribution":0.62147020895873,"citation_network_contribution":0.0,"self_endowment_contribution":0.62147020895873,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":62,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1774677,"name":"Bernd W. Koenig","orcid":null,"position":1,"is_corresponding":false},{"id":583140,"name":"Dieter Willbold","orcid":"0000-0002-0065-7366","position":2,"is_corresponding":false},{"id":1774675,"name":"Julian M. Glück","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Nanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies","abstract":"Nanodiscs are small-sized and flat model membranes that provide a close to native environment for reconstitution of integral membrane proteins. Incorporation of membrane proteins into nanodiscs results in water-soluble proteolipid particles making the membrane proteins amenable to a multitude of bioanalytical techniques originally developed for soluble proteins. The transmembrane domain of the human CD4 receptor was fused to ubiquitin with a preceding N-terminal decahistidine tag. The resulting integral membrane protein was incorporated into nanodiscs. Binding of the nanodisc-inserted histidine-tagged protein to a monoclonal anti-pentahistidine antibody was quantified using surface plasmon resonance (SPR) experiments. For the first time, a membrane-inserted transmembrane protein was employed as analyte while the antibody served as ligand immobilized on the sensor chip surface. SPR experiments were conducted in single-cycle mode. We demonstrate that the nanodisc-incorporated membrane protein showed nearly identical affinity toward the antibody as did the soluble decahistidine-tagged ubiquitin studied in a comparative experiment. Advantages of the new experimental setup and potential applications are discussed.","is_dataset_classified":null,"base_score":4.143134726391533,"endowment":4.143134726391533,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20804721","pmcid":null,"openalex_id":"https://openalex.org/W2026240692","authors":[],"funders":[],"total_grants":0,"fwci":2.1167,"citation_percentile":0.86751922,"influential_citations":0,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":7},{"year":2014,"count":6},{"year":2015,"count":4},{"year":2016,"count":5},{"year":2017,"count":8},{"year":2018,"count":2},{"year":2019,"count":6},{"year":2020,"count":3},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2023,"count":4},{"year":2025,"count":2},{"year":2026,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0003269710005427?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0003269710005427?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ab.2010.08.028","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20804721","host_type":"repository"},{"url":"http://juser.fz-juelich.de/search?p=id:%22PreJuSER-11819%22","host_type":"repository"}],"fields_of_study":["Advanced biosensing and bioanalysis techniques","Lipid Membrane Structure and Behavior","Monoclonal and Polyclonal Antibodies Research"],"mesh_terms":["Antibodies, Monoclonal","Humans","Kinetics","Membrane Proteins","Oligopeptides","Protein Binding","Recombinant Fusion Proteins","Biosensing Techniques","CD4 Antigens","Surface Plasmon Resonance","Ubiquitin","Nanostructures"],"keywords":["Nanodisc","Integral membrane protein","Membrane protein","Transmembrane protein","Surface plasmon resonance","Chemistry","Membrane","Biophysics","Lipid bilayer","Analyte","Transmembrane domain","Biochemistry","Nanoparticle","Nanotechnology","Materials science","Chromatography","Biology","Receptor"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T12:36:40.071414Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}