{"doi":"10.1016/0378-1119(85)90120-9","title":"Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors","abstract":"Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors. Mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences. A new suppressorless strain facilitates the cloning of selected recombinants. (2) The complete nucleotide sequences of the M13mp and pUC vectors have been compiled from a number of sources, including the sequencing of selected segments. The M13mp18 sequence is revised to include the G-to-T substitution in its gene II at position 6 125 bp (in M13) or 6967 bp in M13mp18. (3) M13 clones suitable for sequencing have been obtained by a new method of generating unidirectional progressive deletions from the polycloning site using exonucleases HI and VII.","journal":"Gene","year":1985,"id":5294,"datarank":1.4439821364924375,"base_score":9.626547576616248,"endowment":9.626547576616248,"self_citation_contribution":1.4439821364924375,"citation_network_contribution":0.0,"self_endowment_contribution":1.4439821364924375,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15161,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0651,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"1985-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":52864,"name":"Jeffrey Vieira","orcid":null,"position":1,"is_corresponding":false},{"id":52865,"name":"Joachim Messing","orcid":"0000-0001-8260-2643","position":2,"is_corresponding":false},{"id":52863,"name":"Celeste Yanisch-Perron","orcid":null,"position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}