{"doi":"10.1007/s42995-024-00220-6","title":"Spontaneous mutations and mutational responses to penicillin treatment in the bacterial pathogen Streptococcus pneumoniae D39","abstract":"Abstract Bacteria with functional DNA repair systems are expected to have low mutation rates due to strong natural selection for genomic stability. However, our study of the wild-type Streptococcus pneumoniae D39, a pathogen responsible for many common diseases, revealed a high spontaneous mutation rate of 0.02 per genome per cell division in mutation-accumulation (MA) lines. This rate is orders of magnitude higher than that of other non-mutator bacteria and is characterized by a high mutation bias in the A/T direction. The high mutation rate may have resulted from a reduction in the overall efficiency of selection, conferred by the tiny effective population size in nature. In line with this, S. pneumoniae D39 also exhibited the lowest DNA mismatch-repair (MMR) efficiency among bacteria. Treatment with the antibiotic penicillin did not elevate the mutation rate, as penicillin did not induce DNA damage and S. pneumoniae lacks a stress response pathway. Our findings suggested that the MA results are applicable to within-host scenarios and provide insights into pathogen evolution.","journal":"Marine Life Science & Technology","year":2024,"id":465309,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9548,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1020914,"name":"Tongtong Lin","orcid":"0000-0002-3515-2018","position":1,"is_corresponding":false},{"id":420727,"name":"Jiao Pan","orcid":"0000-0003-3849-2891","position":2,"is_corresponding":false},{"id":1088139,"name":"Caitlyn E. Rivera","orcid":null,"position":3,"is_corresponding":false},{"id":1088138,"name":"Clayton Tincher","orcid":null,"position":4,"is_corresponding":false},{"id":874407,"name":"Yaohai Wang","orcid":null,"position":5,"is_corresponding":false},{"id":1296380,"name":"Yu Zhang","orcid":"0000-0001-7378-6946","position":6,"is_corresponding":false},{"id":266726,"name":"Xiang Gao","orcid":"0000-0001-6397-5639","position":7,"is_corresponding":false},{"id":1296381,"name":"Yan Wang","orcid":"0009-0006-7729-263X","position":8,"is_corresponding":false},{"id":469000,"name":"Ho‐Ching Tiffany Tsui","orcid":"0000-0003-0849-874X","position":9,"is_corresponding":false},{"id":405419,"name":"Malcolm E. Winkler","orcid":"0000-0002-1482-2588","position":10,"is_corresponding":false},{"id":337882,"name":"Michael Lynch","orcid":"0000-0002-1653-0642","position":11,"is_corresponding":false},{"id":337879,"name":"Hongan Long","orcid":"0000-0001-9767-8173","position":12,"is_corresponding":false},{"id":1296379,"name":"Wanyue Jiang","orcid":"0000-0002-5670-5953","position":0,"is_corresponding":true}],"reference_count":91,"raw_metadata":null,"created_at":"2026-07-19T02:04:46.201722Z","pmid":"38827133","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}