{"doi":"10.1007/s13205-021-02927-0","title":"Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H","abstract":null,"journal":"3 Biotech","year":2021,"id":649486,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1693141,"name":"Changfeng Qu","orcid":null,"position":1,"is_corresponding":false},{"id":1693142,"name":"Jinlai Miao","orcid":null,"position":2,"is_corresponding":false},{"id":1693143,"name":"Zhenxia Sha","orcid":null,"position":3,"is_corresponding":false},{"id":1693139,"name":"Meiling An","orcid":"0000-0002-4265-5043","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H","abstract":"Two gene of class II photolyases, <i>PiPhr1</i> (1833 bp) and <i>PiPhr2</i> (1809 bp), from the Antarctic diatom <i>Phaeodactylum tricornutum</i> ICE-H were cloned, the recombinant proteins expressed and purified. The molecular weight of the recombinant photolyases were determined to be 68 kDa with a pI of 9.04 and 68.82 with a pI of 7.31, respectively. Activity studies showed that both the recombinant enzymes were involved in the repair DNA damaged by UV light, that is they were most likely photolyases involved in photorepair of DNA. Further confirmation of this function was demonstrated by the increased expression of <i>PiPhr1</i> and <i>PiPhr2</i> after exposure to UV radiation, blue light and dark conditions by qRT-PCR. In summary, <i>PiPhr1</i> and <i>PiPhr2</i> were up regulated by UVB irradiation and blue light at 0.5 h and 3 h. Longtime (3 h) exposure to dark also increased the expression of <i>PiPhr1</i> and <i>PiPhr2</i>. In vitro photoreactivation assays showed that <i>PiPhr1</i> and <i>PiPhr2</i> could repair CPDs utilizing blue light. This is the first time CPD Class II photolyase has been reported from Antarctic diatom. These results will add to the knowledge of the diatom CPF family and assist in understanding the functional role of these genes in Antarctic diatoms.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13205-021-02927-0.","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34367869","pmcid":"PMC8295441","openalex_id":"https://openalex.org/W3184172712","authors":[],"funders":[{"funder_name":"National Key Research and Development Program of China","grant_id":"2018YFD0900705","title":null},{"funder_name":"Basic Scientific Fund for National Public Research Institutes of China","grant_id":"2020Q02","title":null},{"funder_name":"Natural Science Foundation of China","grant_id":"32000074","title":null},{"funder_name":"Natural Science Foundation of Shandong","grant_id":"ZR2019BD023","title":null},{"funder_name":"tai mountain industry leading talent of shan dong","grant_id":"2019TSCYCX-06","title":null},{"funder_name":"China Postdoctoral Science Foundation","grant_id":"2019M662295","title":null},{"funder_name":"Postdoctoral Applied Research Projects of Qingdao","grant_id":"QD2019013","title":null}],"total_grants":7,"fwci":1.4847,"citation_percentile":0.84043169,"influential_citations":0,"citation_trend":[{"year":2022,"count":5},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":"https://www.springer.com/tdm","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8295441","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8295441","host_type":"repository"},{"url":"https://link.springer.com/content/pdf/10.1007/s13205-021-02927-0.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1007/s13205-021-02927-0/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1007/s13205-021-02927-0","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34367869","host_type":"repository"}],"fields_of_study":["Light effects on plants","Algal biology and biofuel production","Photosynthetic Processes and Mechanisms"],"mesh_terms":[],"keywords":["Photolyase","Phaeodactylum tricornutum","Diatom","Recombinant DNA","Biochemistry","Biology","PMSF","Molecular biology","DNA","Enzyme","Gene","DNA repair","Chemistry","Botany","CPD","photoreactivation","Cpd Photolyase","Antarctic Diatom P. Tricornutum Ice-h"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T03:48:16.602813Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}