{"doi":"10.1007/s12975-020-00800-5","title":"Perlecan Domain-V Enhances Neurogenic Brain Repair After Stroke in Mice","abstract":"The extracellular matrix fragment perlecan domain V is neuroprotective and functionally restorative following experimental stroke. As neurogenesis is an important component of chronic post-stroke repair, and previous studies have implicated perlecan in developmental neurogenesis, we hypothesized that domain V could have a broad therapeutic window by enhancing neurogenesis after stroke. We demonstrated that domain V is chronically increased in the brains of human stroke patients, suggesting that it is present during post-stroke neurogenic periods. Furthermore, perlecan deficient mice had significantly less neuroblast precursor cells after experimental stroke. Seven-day delayed domain V administration enhanced neurogenesis and restored peri-infarct excitatory synaptic drive to neocortical layer 2/3 pyramidal neurons after experimental stroke. Domain V's effects were inhibited by blockade of α2β1 integrin, suggesting the importance of α2β1 integrin to neurogenesis and domain V neurogenic effects. Our results demonstrate that perlecan plays a previously unrecognized role in post-stroke neurogenesis and that delayed DV administration after experimental stroke enhances neurogenesis and improves recovery in an α2β1 integrin-mediated fashion. We conclude that domain V is a clinically relevant neuroprotective and neuroreparative novel stroke therapy with a broad therapeutic window.","journal":"Translational Stroke Research","year":2020,"id":97690,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9524,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":482986,"name":"Michael Kahle","orcid":"0000-0002-5982-3832","position":1,"is_corresponding":false},{"id":467976,"name":"Jill Roberts","orcid":"0000-0002-0799-6934","position":2,"is_corresponding":false},{"id":483905,"name":"Aileen Marcelo","orcid":null,"position":3,"is_corresponding":false},{"id":483906,"name":"Leon de Hoog","orcid":null,"position":4,"is_corresponding":false},{"id":482987,"name":"Jeffery A. Boychuk","orcid":"0000-0002-7003-1553","position":5,"is_corresponding":false},{"id":299458,"name":"Stephen Grupke","orcid":"0000-0002-7170-6081","position":6,"is_corresponding":false},{"id":482988,"name":"Antonio Berretta","orcid":"0000-0003-0216-3543","position":7,"is_corresponding":false},{"id":483907,"name":"Emma K. Gowing","orcid":null,"position":8,"is_corresponding":false},{"id":482989,"name":"Carie R. Boychuk","orcid":"0009-0000-1600-7301","position":9,"is_corresponding":false},{"id":483908,"name":"Amanda A. Gorman","orcid":null,"position":10,"is_corresponding":false},{"id":482990,"name":"Danielle N. Edwards","orcid":"0000-0001-5087-0029","position":11,"is_corresponding":false},{"id":482991,"name":"Ibolya Rutkai","orcid":"0000-0001-6974-6386","position":12,"is_corresponding":false},{"id":482992,"name":"Ifechukwude Joachim Biose","orcid":"0000-0001-8898-4566","position":13,"is_corresponding":false},{"id":482993,"name":"Hatsue Ishibashi‐Ueda","orcid":"0000-0001-5153-9805","position":14,"is_corresponding":false},{"id":482994,"name":"Masafumi Ihara","orcid":"0000-0002-7102-4048","position":15,"is_corresponding":false},{"id":482995,"name":"Bret N. Smith","orcid":"0000-0003-4743-1564","position":16,"is_corresponding":false},{"id":482996,"name":"Andrew N. Clarkson","orcid":"0000-0003-3804-3834","position":17,"is_corresponding":false},{"id":291402,"name":"Gregory Bix","orcid":"0000-0002-8969-9553","position":18,"is_corresponding":false},{"id":467975,"name":"Amanda L. Trout","orcid":"0000-0002-4754-8634","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-18T22:36:02.818637Z","pmid":"32253702","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}