{"doi":"10.1007/s12551-010-0032-5","title":"Protein structural dynamics revealed by site-directed spin labeling and multifrequency EPR","abstract":null,"journal":"Biophysical Reviews","year":2010,"id":659116,"datarank":0.5333022092234121,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"self_citation_contribution":0.5333022092234121,"citation_network_contribution":0.0,"self_endowment_contribution":0.5333022092234121,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":417547,"name":"David D. Thomas","orcid":"0000-0002-8822-2040","position":1,"is_corresponding":false},{"id":580668,"name":"Yuri E. Nesmelov","orcid":"0000-0003-0191-2274","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Protein structural dynamics revealed by site-directed spin labeling and multifrequency EPR","abstract":"Multifrequency electron paramagnetic resonance (EPR), combined with site-directed spin labeling, is a powerful spectroscopic tool to characterize protein dynamics. The lineshape of an EPR spectrum reflects combined rotational dynamics of the spin probe's local motion within a protein, reorientations of protein domains, and overall protein tumbling. All these motions can be restricted and anisotropic, and separation of these motions is important for thorough characterization of protein dynamics. Multifrequency EPR distinguishes between different motions of a spin-labeled protein, due to the frequency dependence of EPR resolution to fast and slow motion of a spin probe. This gives multifrequency EPR its unique capability to characterize protein dynamics in great detail. In this review, we analyze what makes multifrequency EPR sensitive to different rates of spin probe motion and discuss several examples of its usage to separate spin probe dynamics and overall protein dynamics, to characterize protein backbone dynamics, and to resolve protein conformational states.","is_dataset_classified":null,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21687819","pmcid":"PMC3115708","openalex_id":"https://openalex.org/W2166741831","authors":[],"funders":[{"funder_name":"NIAMS NIH HHS","grant_id":"K25 AR053562","title":null},{"funder_name":"NIAMS NIH HHS","grant_id":"R37 AR032961","title":null},{"funder_name":"NIAMS NIH HHS","grant_id":"R01 AR032961","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01AR032961-30","title":"Molecular Dynamics of Muscle Contraction"}],"total_grants":4,"fwci":2.3465,"citation_percentile":0.89004914,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":3},{"year":2014,"count":5},{"year":2015,"count":1},{"year":2016,"count":2},{"year":2017,"count":4},{"year":2018,"count":1},{"year":2019,"count":4},{"year":2020,"count":4},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"green","license":"Springer TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3115708","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3115708","host_type":"repository"},{"url":"http://link.springer.com/content/pdf/10.1007/s12551-010-0032-5.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s12551-010-0032-5/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s12551-010-0032-5","host_type":"publisher"},{"url":"https://doi.org/10.1007/s12551-010-0032-5","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21687819","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115708","host_type":""},{"url":"https://dx.doi.org/10.1007/s12551-010-0032-5","host_type":""}],"fields_of_study":["Electron Spin Resonance Studies","Lanthanide and Transition Metal Complexes","Advanced NMR Techniques and Applications","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Electron paramagnetic resonance","Site-directed spin labeling","Protein dynamics","Dynamics (music)","Spin label","Chemistry","Spin (aerodynamics)","Chemical physics","Spin probe","Molecular dynamics","Pulsed EPR","Nuclear magnetic resonance","Physics","Spin echo","Computational chemistry","Magnetic resonance imaging"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T06:12:59.200899Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}