{"doi":"10.1007/s12230-023-09922-6","title":"Culture-based Determination of Verticillium Densities in Soil Overestimates Disease Pressure of Verticillium Wilt of Potato in Manitoba","abstract":"<jats:title>Abstract</jats:title><jats:p>Verticillium wilt affects potato fields in Manitoba where 80% of the fields are planted to the moderately susceptible cultivar, Russet Burbank. An accurate determination of <jats:italic>Verticillium dahliae</jats:italic> inoculum in soil is critical for disease management. In this study, we investigated the presence of microsclerotia-producing <jats:italic>Verticillium</jats:italic> species in potato fields in Manitoba and compared published quantitative real-time PCR assays for <jats:italic>V. dahliae, V. tricorpus</jats:italic> and <jats:italic>V. longisporum</jats:italic> against a traditional plating method. Selected real-time PCR assays could differentiate and quantify the major microsclerotia-producing species, <jats:italic>V. dahliae</jats:italic>, <jats:italic>V. tricorpus</jats:italic> and <jats:italic>V. longisporum</jats:italic>. Results showed that the presence of <jats:italic>V. tricorpus</jats:italic> caused an overestimation of <jats:italic>V. dahliae</jats:italic> propagule density when using the plating method. As a result, Verticillium wilt severity was negatively related to cfu from traditional plating, while positive with the amounts of <jats:italic>V. dahliae</jats:italic> genomic DNA in soils.</jats:p>","journal":"American Journal of Potato Research","year":2023,"id":43038,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":205112,"name":"Maria A. Henriquez","orcid":null,"position":1,"is_corresponding":false},{"id":205113,"name":"Mario Tenuta","orcid":null,"position":2,"is_corresponding":false},{"id":205111,"name":"Oscar I. Molina","orcid":"0009-0005-4318-4721","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18998881","pmcid":null,"openalex_id":"https://openalex.org/W4386199488","authors":[],"funders":[],"total_grants":0,"fwci":0.3838,"citation_percentile":0.63083509,"influential_citations":0,"citation_trend":[{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/s12230-023-09922-6.pdf","host_type":"journal"},{"url":"https://link.springer.com/content/pdf/10.1007/s12230-023-09922-6.pdf","host_type":"HYBRID"},{"url":"https://link.springer.com/content/pdf/10.1007/s12230-023-09922-6.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1007/s12230-023-09922-6/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1007/s12230-023-09922-6","host_type":"journal"},{"url":"https://open-science.canada.ca/handle/123456789/3184","host_type":"repository"},{"url":"https://open-science.canada.ca/server/api/core/bitstreams/08b4d776-4a4b-48fa-a9e7-61f47bdd4a2a/content","host_type":"repository"}],"fields_of_study":["Plant Pathogens and Fungal Diseases","Plant-Microbe Interactions and Immunity","Plant Disease Resistance and Genetics","Agricultural and Food Sciences","Environmental Science"],"mesh_terms":[],"keywords":["Verticillium dahliae","Verticillium wilt","Verticillium","Biology","Cultivar","Horticulture","Wilt disease","Agronomy","Botany"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T13:46:04.144184Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}